LOCUS       sequence-110792-        1059 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Codon-optimized human dynein-2 motor domain (D1091–Q4307) for 
            baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to 
            cleave 6His-ZZ..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Schmidt H, Zalyte R, Urnavicius L, Carter AP
  TITLE     Structure of human cytoplasmic dynein-2 primed for its power stroke.
  JOURNAL   Nature. 2015 Feb 19;518(7539):435-8. doi: 10.1038/nature14023. Epub 
            2014 Dec 1.
  PUBMED    25470043
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14023"; journalName: "Nature"; date: "2015-02-19- 
            19"; volume: "518"; issue: "7539"; pages: "435-8"
COMMENT     Sequence Label: pFastBac-GFP-dynein-2(D1091–Q4307)
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             111..134
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             177..350
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAYYEILHLPNLNEGQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             351..524
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAEKLNDAQAPK"
     CDS             582..602
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             624..644
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatattccg gattattcat
       61 accgtcccac catcgggcgc ggatctcggt ccgaaaccat gtcgtactac catcaccatc
      121 accatcacca tcacggttct atgtgcatgg caggccttgc gcaacacgat gaagccgtgg
      181 acaacaaatt caacaaagaa caacaaaacg cgtactatga gatcttacat ttacctaact
      241 taaacgaagg acaacgaaac gccttcatcc aaagtttaaa agatgaccca agccaaagcg
      301 ctaacctttt agcagaagct aaaaagctaa atgatgctca ggcgccgaaa gtagacaaca
      361 aattcaacaa agaacaacaa aacgcgttct atgagatctt acatttacct aacttaaacg
      421 aagaacaacg aaacgccttc atccaaagtt taaaagatga cccaagccaa agcgctaacc
      481 ttttagcaga agctgaaaag ctaaatgatg ctcaggcgcc gaaagtagac gcaaattccg
      541 cggggaagtc aaccgattat gatattccaa ctactgctag cgagaaccta tatttccagg
      601 gagattatga tatccctacg actgagaatc tttattttca gggcatgtct aaaggtgaag
      661 aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt aatggtcaca
      721 aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg accttaaaat
      781 ttatttgtac tactggtaaa ttgccagttc catggccaac cttagtcact actttcggtt
      841 atggtgttca atgttttgcg agatacccag atcatatgaa acaacatgac tttttcaagt
      901 ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat gacggtaact
      961 acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga atcgaattaa
     1021 aaggtattga ttttaaagaa gatggtaaca ttttaggtc
//
