LOCUS       sequence-111033-         745 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Targeting vector for the human AAVS1 locus for insertion of traffic 
            light reporter (TLR) construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
  TITLE     Increasing the efficiency of homology-directed repair for 
            CRISPR-Cas9-induced precise gene editing in mammalian cells.
  JOURNAL   Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
  PUBMED    25803306
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198."
COMMENT     Sequence Label: pAAVS1-TLR targeting vector
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..59)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(71..322)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(113..134)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     462..481
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     721..742
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ataggcccgg ggttttcttc aacatctcct gcttgcttta acagagagaa gttcgtggcc
       61 aacgcgtcta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
      121 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactggtagc
      181 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      241 agtggtcggc gagctgcacg ccgccgtcct cgatgttgtg gcggatcttg aagttggcct
      301 tgatgccgtt cttctgcttg tcggcggtga tcaccaatgg tggcccggta gtgcgaggag
      361 aagttttggt gcacatagcg cttgatataa gcctgcccag aagactcccg cccatcttct
      421 agaaagactg gagttgcagc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
      481 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
      541 cgtgctgctt catgtggtcg gggtagcggg cgaagcactg caggccgtag cccagggtgg
      601 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatcagc ttcagggtca
      661 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
      721 cgtcgccgtc cagctcgacc aggat
//
