LOCUS       sequence-111211-         966 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  eukaryotic expression plasmid for VZV glycoprotein L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Birlea M, Owens GP, Eshleman EM, Ritchie A, Traktinskiy I, Bos N, 
            Seitz S, Azarkh Y, Mahalingam R, Gilden D, Cohrs RJ
  TITLE     Human anti-varicella-zoster virus (VZV) recombinant monoclonal 
            antibody produced after Zostavax immunization recognizes the gH/gL 
            complex and neutralizes VZV infection.
  JOURNAL   J Virol. 2013 Jan;87(1):415-21. doi: 10.1128/JVI.02561-12. Epub 2012
            Oct 17.
  PUBMED    23077312
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02561-12"; journalName: "J Virol"; date: "2013-01"; 
            volume: "87"; issue: "1"; pages: "415-21"
COMMENT     Sequence Label: VZV ORF 60 (gL)
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          168..300
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     283..302
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        345..363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             852..869
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcgagctcg ttagtgaacc gtcagatcac tagaagcttt attgcggtag tttatcacag
       61 ttaaattgct aacgcagtca gtgcttctga cacaacagtc tcgaacttaa gctgcagtga
      121 ctctcttaag gtagccttgc agaagttggt cgtgaggcac tgggcaggta agtatcaagg
      181 ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct
      241 tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag
      301 gtgtccactc ccagttcaat tacagctctt aaggctagag tacttaatac gactcactat
      361 aggctagcat ggcatcacat aaatggttac tgcagatagt ttttttaaaa actatcacaa
      421 tcgcgtattg tcttcatctc caagacgaca ctccgttgtt ttttggagcc aaaccgctat
      481 cggatgtgag tttgattata acggaaccgt gcgtgtcatc ggtatatgag gcgtgggact
      541 atgcggcacc cccggtatca aacctcagcg aggcgctatc gggaatcgtg gttaagacaa
      601 aatgtccagt accggaagtt atactttggt ttaaagacaa acaaatggcg tactggacaa
      661 atccatacgt caccttaaag gggctggcac aatctgttgg tgaagaacat aaaagcgggg
      721 acatacgcga tgctttgttg gatgcccttt ccggtgtatg ggtagactct actccatctt
      781 ccacaaatat cccggaaaat ggatgtgtct ggggagccga ccgtttgttc caacgcgtat
      841 gccaactcga gcaccatcac caccatcatt gagtcgactg agcggccgct tccctttagt
      901 gagggttaat gcttcgagca gacatgataa gatacattga tgagtttggg acaaaccaca
      961 actaga
//
