LOCUS       sequence-111215-         737 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Initial Integrative Vector
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, 
            Patron-Soberano A, De Las Penas A, Castano I
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in Candida glabrata.
  JOURNAL   Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020.
  PUBMED    25986172
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020."
COMMENT     Sequence Label: pYC44
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    55..88
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      complement(131..328)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga ggacgaagtt
       61 cctattctct agaaagtata ggaacttcgt cgatcgacac tggatggcgg cgttagtatc
      121 gaatcgacag cagtatagcg accagcattc acatacgatt gacgcatgat attactttct
      181 gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg aaaaaaaata taaatcacgc
      241 taacatttga ttaaaataga acaactacaa tataaaaaaa ctatacaaat gacaagttct
      301 tgaaaacaag aatcttttta ttgtcagtac tggtcgagtt attatggaca tggcatagac
      361 atatacaaag cttgttcacc atcggaagca gtaccatcgt ataaagcagt atccaaacca
      421 cacaaagtga aacccattct tctataagca tgaatagctg gagcattaac attggtaact
      481 tccaaccaca aatgaccagc acctctttct ctggcgaatt cagtagccaa acccatcaaa
      541 gctctaccaa caccatgacc tctatgttct ggagcaactt caatatcttc aacagtcaat
      601 cttctgttcc aaccagaata agaaacaaca acgaaaccag ccaaatcacc atcatcacca
      661 taagcaacga aagttctaga atctggatca ccatcttcac cagcatcgga ttcatcatcg
      721 gattcatcat ctgggaa
//
