LOCUS       sequence-111517-         840 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  encodes c2 gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pRPR1_c2gRNA_RPR1t
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(351..413)
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
     misc_RNA        complement(426..501)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tagtggaaca aaagcaaatg aaggatttca tgcgtttgta ctctaatctg gtagaaagat
       61 gtttcacaga ctgtgtcaat gacttcacaa catcaaagct aaccaataag gaacaaacat
      121 gcatcatgaa gtgctcagaa aagttcttga agcatagcga acgtgtaggg cagcgtttcc
      181 aagaacaaaa cgctgccttg ggacaaggct tgggccgata aggtgtactg gcgtatatat
      241 atctaattat gtatctctgg tgtagcccat ttttagcatg taaatataaa gagaaaccat
      301 atctaatcta accaaatcca aacaaaattc aatagttact atcgcttttt tctttctgta
      361 tcgcaaataa gtgaaaatta aaaaagaaag attaaattgg aagttggata tggctcgaga
      421 aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattttaact
      481 tgctatttct agctctaaaa caatgtataa ggaaagaata taagcttctg ccaatcgcag
      541 ctcccagagt ttcgttccca gccgcgagca ccacagcgta ccatgtgcgc cacgaggcct
      601 caaacgtaaa acaatcgaaa cgaaaagaaa cagactatag gggagtatag agacagccgg
      661 ccaataagaa gaggaaaaag aaatactagc gtttatcaat gtgggtcgtt aacatatcct
      721 gttgacaatg attacaggtt aaaaggtagc gtaagtgaat attaactatg gatattctta
      781 tacttagata agagacatat aaaacacacg atgattgatt gatttttatg accaatatat
//
