LOCUS       sequence-111531-         838 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses GST-Ago2 S387A mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, 
            Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, 
            Novina CD, Orth AP
  TITLE     Akt-mediated phosphorylation of argonaute 2 downregulates cleavage 
            and upregulates translational repression of MicroRNA targets.
  JOURNAL   Mol Cell. 2013 May 9;50(3):356-67. doi: 
            10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18.
  PUBMED    23603119
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.03.015"; journalName: "Mol Cell"; date: 
            "2013-05-9- 9"; volume: "50"; issue: "3"; pages: "356-67"
COMMENT     Sequence Label: pGEX 4T-1 GST-Ago2 S387A
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..25
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atcggatctg gttccgcgtg gatccccgga attctactcg ggagccggcc ccgcacttgc
       61 acctcctgcg ccgccgcccc ccatccaagg atatgccttc aagcctccac ctagacccga
      121 ctttgggacc tccgggagaa caatcaaatt acaggccaat ttcttcgaaa tggacatccc
      181 caaaattgac atctatcatt atgaattgga tatcaagcca gagaagtgcc cgaggagagt
      241 taacagggaa atcgtggaac acatggtcca gcactttaaa acacagatct ttggggatcg
      301 gaagcccgtg tttgacggca ggaagaatct atacacagcc atgccccttc cgattgggag
      361 ggacaaggtg gagctggagg tcacgctgcc aggagaaggc aaggatcgca tcttcaaggt
      421 gtccatcaag tgggtgtcct gcgtgagctt gcaggcgtta cacgatgcac tttcagggcg
      481 gctgcccagc gtcccttttg agacgatcca ggccctggac gtggtcatga ggcacttgcc
      541 atccatgagg tacacccccg tgggccgctc cttcttcacc gcgtccgaag gctgctctaa
      601 ccctcttggc gggggccgag aagtgtggtt tggcttccat cagtccgtcc ggccttctct
      661 ctggaaaatg atgctgaata ttgatgtgtc agcaacagcg ttttacaagg cacagccagt
      721 aatcgagttt gtttgtgaag ttttggattt taaaagtatt gaagaacaac aaaaacctct
      781 gacagattcc caaagggtaa agtttaccaa agaaattaaa ggtctaaagg tggagata
//
