LOCUS       sequence-111686-        9696 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9696)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 9696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pT2-7xTcf-NLS-CNL
FEATURES             Location/Qualifiers
     source          1..9696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2123..2171
                     /note="synthetic polyadenylation signal"
     primer_bind     2346..2370
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2460..2480
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2484..2504
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2508..2528
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2541..2543,2544..2546,2547..3059)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(2586..2607)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2847..2866)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3194..3215
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4220..4444
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4491..4514)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4888..4907
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4972..5571
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4972..4991)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5468..5488
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5658..5882
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5910..5926)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5934..5950
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5958..5964,5965..5982,5983..5988))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6003..6024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        complement(7508..7526)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7547..7563)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7571..7587
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7595..7601,7602..7619,7620..7625))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7640..7661
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7778..7795)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7949..8537)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8029..8048)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8708..9499,9500..9568))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9331..9350
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9569..9673)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccagagg
      661 tgtaaagtac ttgagtaatt ttacttgatt actgtactta agtattattt ttggggattt
      721 ttactttact tgagtacaat taaaaatcaa tacttttact tttacttaat tacatttttt
      781 tagaaaaaaa agtacttttt actccttaca attttattta cagtcaaaaa gtacttattt
      841 tttggagatc acttgtcgag tagggataac agggtaatga tcccccgggc tgcagtcgcg
      901 acgcgtccta gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc
      961 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca
     1021 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
     1081 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa
     1141 aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc ctgggttgca
     1201 aaggagcaca gtgctcatcc agatccaacc ccctgctatg tgcagggtca tcaaccagca
     1261 gcccaggctg cccagagcca catccagcct ggccttgaat gcctgcaggg atggggcatc
     1321 cacagcctcc ttgggcaacc tgttcagtgc gtcaccaccc tctgggggaa aaactgcctc
     1381 ctcatatcca acccaaacct cccctgtctc agtgtaaagc cattccccct tgtcctatca
     1441 agggggagtt tgctgtgaca ttgttggtct ggggtgacac atgtttgcca attcagtgca
     1501 tcacggagag gcagatcttg gggataagga agtgcaggac agcatggacg tgggacatgc
     1561 aggtgttgag ggctctggga cactctccaa gtcacagcgt tcagaacagc cttaaggata
     1621 agaagatagg atagaaggac aaagagcaag ttaaaaccca gcatggagag gagcacaaaa
     1681 aggccacaga cactgctggt ccctgtgtct gagcctgcat gtttgatggt gtctggatgc
     1741 aagcagaagg ggtggaagag cttgcctgga gagatacagc tgggtcagta ggactgggac
     1801 aggcagctgg agaattgcca tgtagatgtt catacaatcg tcaaatcatg aaggctggaa
     1861 aagccctcca agatccccaa gaccaacccc aacccaccca ccgtgcccac tggccatgtc
     1921 cctcagtgcc acatccccac agttcttcat cacctccagg gacggtgacc cccccacctc
     1981 cgtgggcagc tgtgccactg cagcaccgct ctttggagaa ggtaaatctt gctaaatcca
     2041 gcccgaccct cccctggcac aacgtaaggc cattatctct catccaactc caggacggag
     2101 tcagtgagga tggggctcta ggaataaaat atctttattt tcattacatc tgtgtgttgg
     2161 ttttttgtgt ggtcgacggt accgagctct tacgcgagat caaagggggt aagatcaaag
     2221 ggggtaagat caaaggggcg cgagatcaaa gggggtaaga tcaaaggggg taagatcaaa
     2281 gggggtaaga tcaaaggggc gcgcccgcgt gctaggcgtg tacggtggga ggcctatata
     2341 agcagagctc gtttagtgaa ccgtcagatc gcatcgattc gaattccagc ttggcattcc
     2401 ggtactgttg gtaaaaagct tggcattccg gtactgttgg taaagccacc atgctggtac
     2461 caaaaaagaa gagaaaggtt gtaccaaaaa agaagagaaa ggttgtacca aaaaagaaga
     2521 gaaaggtttt cgaagggccc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     2581 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     2641 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     2701 tgcccgtgcc ctggcccacc ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc
     2761 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     2821 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2881 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2941 acggcaacat cctggggcac aagctggagt acaactactt tagcgacaac gtctatatca
     3001 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
     3061 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     3121 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
     3181 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggggt accaaggtgt
     3241 acgaccccga gcagaggaag aggatgatca ccggccccca gtggtgggcc aggtgcaagc
     3301 agatgaacgt gctggacagc ttcatcaact actacgacag cgagaagcac gccgagaacg
     3361 ccgtgatctt cctgcacggc aacgccacta gcagctacct gtggaggcac gtggtgcccc
     3421 acatcgagcc cgtggccagg tgcatcatcc ccgatctgat cggcatgggc aagagcggca
     3481 agagcggcaa cggcagctac aggctgctgg accactacaa gtacctgacc gcctggttcg
     3541 agcttctgaa cctgcccaag aagatcatct tcgtgggcca cgactggggc gccgccctgg
     3601 ccttccacta cgcctacgag caccaggaca ggatcaaggc catcgtgcac atggagagcg
     3661 tggtggacgt gatcgagagc tgggacgagt ggccagacat cgaggaggac atcgccctga
     3721 tcaagagcga ggagggcgag aagatggtgc tggagaacaa cttcttcgtg gagaccgtgc
     3781 tgcccagcaa gatcatgaga aagctggagc ccgaggagtt cgccgcctac ctggagccct
     3841 tcaaggagaa gggcgaggtg agaagaccca ccctgagctg gcccagagag atccccctgg
     3901 tgaagggcgg caagcccgac gtggtgcaga tcgtgagaaa ctacaacgcc tacctgagag
     3961 ccagcgacga cctgcccaag ctgttcatcg agggcgaccc cggcttcttc agcaacgcca
     4021 tcgtggaggg cgccaagaag ttccccaaca ccgagttcgt gaaggtgaag ggcctgcact
     4081 tcctccagga ggacgccccc gacgagatgg gcaagtacat caagagcttc gtggagagag
     4141 tgctgaagaa cgagcagtaa tctagagcgg ccgctcgata agcttgatcg atgatctaga
     4201 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     4261 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     4321 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     4381 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     4441 atggcttctg aggcggaaag aacctgcagc ttttcccaag gcagtctgga gcatgcgctt
     4501 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     4561 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     4621 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     4681 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     4741 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     4801 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     4861 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     4921 cgctgttctc ctcttcctca tctccgggcc tttcgacctg tggccacaac catgaccgag
     4981 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     5041 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     5101 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     5161 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     5221 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     5281 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     5341 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     5401 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     5461 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     5521 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
     5581 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcgat gatctagagc
     5641 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5701 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5761 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     5821 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     5881 ggctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     5941 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6001 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6061 aacctgtcgt gccagcggat ccctagaggg acagcccccc cccaaagccc ccagggatgt
     6121 aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc
     6181 ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg
     6241 tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg
     6301 gggggatacg gggaaaaagc tttaggctga aagagagatt tagaatgaca gaatcataga
     6361 acggcctggg ttgcaaagga gcacagtgct catccagatc caaccccctg ctatgtgcag
     6421 ggtcatcaac cagcagccca ggctgcccag agccacatcc agcctggcct tgaatgcctg
     6481 cagggatggg gcatccacag cctccttggg caacctgttc agtgcgtcac caccctctgg
     6541 gggaaaaact gcctcctcat atccaaccca aacctcccct gtctcagtgt aaagccattc
     6601 ccccttgtcc tatcaagggg gagtttgctg tgacattgtt ggtctggggt gacacatgtt
     6661 tgccaattca gtgcatcacg gagaggcaga tcttggggat aaggaagtgc aggacagcat
     6721 ggacgtggga catgcaggtg ttgagggctc tgggacactc tccaagtcac agcgttcaga
     6781 acagccttaa ggataagaag ataggataga aggacaaaga gcaagttaaa acccagcatg
     6841 gagaggagca caaaaaggcc acagacactg ctggtccctg tgtctgagcc tgcatgtttg
     6901 atggtgtctg gatgcaagca gaaggggtgg aagagcttgc ctggagagat acagctgggt
     6961 cagtaggact gggacaggca gctggagaat tgccatgtag atgttcatac aatcgtcaaa
     7021 tcatgaaggc tggaaaagcc ctccaagatc cccaagacca accccaaccc acccaccgtg
     7081 cccactggcc atgtccctca gtgccacatc cccacagttc ttcatcacct ccagggacgg
     7141 tgaccccccc acctccgtgg gcagctgtgc cactgcagca ccgctctttg gagaaggtaa
     7201 atcttgctaa atccagcccg accctcccct ggcacaacgt aaggccatta tctctcatcc
     7261 aactccagga cggagtcagt gaggatgggg ctctagccgg accgacgcgt ctcgataggg
     7321 ataacagggt aattgatcca atactcaagt acaattttaa tggagtactt ttttactttt
     7381 actcaagtaa gattctagcc agatactttt acttttaatt gagtaaaatt ttccctaagt
     7441 acttgtactt tcacttgagt aaaatttttg agtacttttt acacctctgg agctccagct
     7501 tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc
     7561 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7621 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7681 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7741 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7801 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7861 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7921 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7981 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8041 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8101 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8161 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8221 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8281 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8341 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     8401 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8461 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8521 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8581 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8641 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8701 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8761 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8821 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8881 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8941 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9001 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9061 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9121 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9181 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9241 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9301 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9361 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9421 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9481 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9541 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9601 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9661 taggggttcc gcgcacattt ccccgaaaag tgccac
//
