LOCUS       sequence-112051-        1781 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses RNA-binding protein MS2 with fast SNAP fusion tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Carrocci TJ, Hoskins AA
  TITLE     Imaging of RNAs in live cells with spectrally diverse small molecule
            fluorophores.
  JOURNAL   Analyst. 2014 Jan 7;139(1):44-7. doi: 10.1039/c3an01550e. Epub 2013 
            Nov 1.
  PUBMED    24187681
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3an01550e"; journalName: "Analyst"; date: "2014-01-7- 7"; 
            volume: "139"; issue: "1"; pages: "44-7"
COMMENT     Sequence Label: MS2-Snapf
FEATURES             Location/Qualifiers
     source          1..1781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             86..106
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             110..136
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             110..136
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             137..148
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             545..1090
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     promoter        1097..1502
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 ttctacttct taattctact tttataggtt agtctttttt ttagttttaa aacaccaaga
       61 acttagtttc gacggatcca ccatgccaaa aaagaaaaga aaagttggct acccctacga
      121 cgtgcccgac tacgccatcg aaggccgcca tatgctagcc gttaaaatgg cttctaactt
      181 tactcagttc gttctcgtcg acaatggcgg aactggcgac gtgactgtcg ccccaagcaa
      241 cttcgctaac gggatcgctg aatggatcag ctctaactcg cgttcacagg cttacaaagt
      301 aacctgtagc gttcgtcaga gctctgcgca gaatcgcaaa tacaccatca aagtcgaggt
      361 gcctaaaggc gcctggcgtt cgtacttaaa tatggaacta accattccaa ttttcgccac
      421 gaattccgac tgcgagctta ttgttaaggc aatgcaaggt ctcctaaaag atggaaaccc
      481 gattccctca gcaatcgcag caaactccgg catctacgcg gccgcaggag gatcaggagg
      541 atcaatggac aaagactgcg aaatgaagcg caccaccctg gatagccctc tgggcaagct
      601 ggaactgtct gggtgcgaac agggcctgca ccgtatcatc ttcctgggca aaggaacatc
      661 tgccgccgac gccgtggaag tgcctgcccc agccgccgtg ctgggcggac cagagccact
      721 gatgcaggcc accgcctggc tcaacgccta ctttcaccag cctgaggcca tcgaggagtt
      781 ccctgtgcca gccctgcacc acccagtgtt ccagcaggag agctttaccc gccaggtgct
      841 gtggaaactg ctgaaagtgg tgaagttcgg agaggtcatc agctacagcc acctggccgc
      901 cctggccggc aatcccgccg ccaccgccgc cgtgaaaacc gccctgagcg gaaatcccgt
      961 gcccattctg atcccctgcc accgggtggt gcagggcgac ctggacgtgg ggggctacga
     1021 gggcgggctc gccgtgaaag agtggctgct ggcccacgag ggccacagac tgggcaagcc
     1081 tgggctgggt taagttaact gtgggaatac tcaggtatcg taagatgcaa gagttcgaat
     1141 ctcttagcaa ccattatttt tttcctcaac ataacgagaa cacacagggg cgctatcgca
     1201 cagaatcaaa ttcgatgact ggaaattttt tgttaatttc agaggtcgcc tgacgcatat
     1261 acctttttca actgaaaaat tgggagaaaa aggaaaggtg agagcgccgg aaccggcttt
     1321 tcatatagaa tagagaagcg ttcatgacta aatgcttgca tcacaatact tgaagttgac
     1381 aatattattt aaggacctat tgttttttcc aataggtggt tagcaatcgt cttactttct
     1441 aacttttctt accttttaca tttcagcaat atatatatat atttcaagga tataccattc
     1501 taatgtctgc ccctaagaag atcgtcgttt tgccaggtga ccacgttggt caagaaatca
     1561 cagccgaagc cattaaggtt cttaaagcta tttctgatgt tcgttccaat gtcaagttcg
     1621 atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtt ccacttccag
     1681 atgaggcgct ggaagcctcc aagaaggctg atgccgtttt gttaggtgct gtgggtggtc
     1741 ctaaatgggg taccggtagt gtagaccgaa caaggtacaa t
//
