LOCUS       sequence-112297-         787 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral expression of murine Flag-Akt2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Kajno E, McGraw TE, Gonzalez E
  TITLE     Development of a new model system to dissect isoform specific Akt 
            signaling in adipocytes.
  JOURNAL   Biochem J. 2015 Apr 9.
  PUBMED    25856301
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2015 Apr 9."
COMMENT     Sequence Label: pLVX-IRES-tdTomato-FlagAkt2
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             105..170
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ctgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
       61 aagacaccga ctctactaga ggatctattt ccggtgaatt catggactac aaagaccatg
      121 acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag cttatgaatg
      181 aggtatctgt catcaaagaa ggctggctcc acaaacgtgg tgaatacatc aagacctgga
      241 ggccacggta cttccttctg aagagtgatg gatctttcat tgggtataag gagaggcccg
      301 aggcccctga ccagacctta ccccccctga acaatttctc tgtagcagaa tgccagctga
      361 tgaagactga gaggccacga cccaacacct ttgtcatacg ctgcctgcag tggaccacag
      421 tcatcgagag gaccttccat gtagactctc cagatgagag ggaagagtgg atgcgggcta
      481 tccagatggt cgccaacagt ctgaagcagc ggggcccagg tgaggacgcc atggattaca
      541 agtgtggctc ccccagtgac tcttccacat ctgagatgat ggaggtagct gtcaacaagg
      601 cacgggccaa agtgaccatg aacgatttcg actatctcaa actcctcggc aagggcacct
      661 tcggcaaggt cattctggtt cgagagaagg ccactggccg ctattatgcc atgaagatcc
      721 tgcgcaagga ggtcatcatt gcaaaggatg aagtcgccca cacagtcaca gagagccggg
      781 ttctgca
//
