LOCUS       sequence-112331-         775 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) 
            in a Cre-dependent manner in an AAV backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Chen Y, Saulnier JL, Yellen G, Sabatini BL
  TITLE     A PKA activity sensor for quantitative analysis of endogenous GPCR 
            signaling via 2-photon FRET-FLIM imaging.
  JOURNAL   Front Pharmacol. 2014 Apr 2;5:56. doi: 10.3389/fphar.2014.00056. 
            eCollection 2014.
  PUBMED    24765076
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Pharmacol."; date: "2014-04-2"; volume: "5:56. doi"; pages: " 
            10.3389/fphar.2014.00056. eCollection 2014"
COMMENT     Sequence Label: AAV-FLEX-PKIalpha-IRES-nls-mRuby2
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    90..123
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgtataaagt atcctatacg aagttatatc aaaataggaa gaccaatgct tcaccatcga
       61 cccgaattgc caagcatcac catcgaccca taacttcgta taatgtatgc tatacgaagt
      121 tatactagga tccccttact tgtacagctc gtccatccca ccaccaagcc cggcgaactt
      181 ggcaactgcg tgttcgcgtt gtactacgaa catttcattg tcactttcct ctaacctttc
      241 caggcggtga tcaacggcat ggataccggg catcttgatg ttcccgacgg tcttttttga
      301 cctgtaagtt gttacgaaag agcaagacag atggccacca ccatcaactt tcagtgccat
      361 atgagtgtat cccctcagac caccatctgc tggatacatc atctctgtat taggctccca
      421 acccttggtc ttcttctgca tcacgggacc attggaggga aagtttaccc ctctgacttg
      481 gacgtggtaa acgagacagc catcctcaag gctggtgtcc tgcatgacgg tgacgactcc
      541 accatcttcg tatctcgtaa ctctttccca agtaaaaccc tcaggaaagg actgtttaaa
      601 gaaatcagga atgcctttcg ggtacttgat aaaagtacgg ctgccataca tgaacgacgt
      661 ggcaagaatg tcaaaggcaa atggcagggg tcctccctcg atgactttga tcctcatggt
      721 ttgagttccc atgtacggat tgccttctcc ttcacctgtg catttgaatt ggtgg
//
