LOCUS       sequence-112486-         851 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + 
            PLtetO-1::micC::Asp terminator + pLtetO-1::RBS (st7) hfq::T1 
            terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL417
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    139..155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        213..317
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(536..555)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 acgcatgcta gctgggacca ggagggacag tactttaact ttcatgttat taaccctctg
       61 ttatatgcct ttatttgctt ttttatgcca ctgcatactg attaaccctc attaatcagt
      121 cggcaagtcg acagtatctt gttatccgct cacaatgtca atgttatccg ctcacattta
      181 tgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      241 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      301 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      361 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      421 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      481 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      541 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
      601 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
      661 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
      721 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
      781 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
      841 agcgtgacac c
//
