LOCUS       sequence-112835-        1004 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  retroviral expression of CCL20 shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Affo S, Morales-Ibanez O, Rodrigo-Torres D, Altamirano J, Blaya D, 
            Dapito DH, Millan C, Coll M, Caviglia JM, Arroyo V, Caballeria J, 
            Schwabe RF, Gines P, Bataller R, Sancho-Bru P
  TITLE     CCL20 mediates lipopolysaccharide induced liver injury and is a 
            potential driver of inflammation and fibrosis in alcoholic 
            hepatitis.
  JOURNAL   Gut. 2014 Nov;63(11):1782-92. doi: 10.1136/gutjnl-2013-306098. Epub 
            2014 Jan 10.
  PUBMED    24415562
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1136/gutjnl-2013-306098"; journalName: "Gut"; date: "2014-11"; 
            volume: "63"; issue: "11"; pages: "1782-92"
COMMENT     Sequence Label: shCCL20
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(52..154)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        275..293
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     ncRNA           complement(371..497)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(523..537)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(563..684)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(739..987)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(797..816)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(967..987)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg acaactcaaa aaatacgccc ggtagtgatc ttatttcatt
      121 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc
      181 ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc
      241 cgtcacaggt atttattcgg tcgaacgcgt aatttaatac gactcactat aggatgttaa
      301 tccacacggc ttgcaccaga gattaaacga caaaatggac tcatacagaa ccacagtctt
      361 ccaattgaaa aaagtgattt aatttatacc attttaattc agctttgtaa aaatgtatca
      421 aagagatagc aaggtattca gttttagtaa acaagataat tgctcctaaa gtagcccctt
      481 gaattccgag gcagtaggca aaagcagaac tgggtgaaaa tacatctgtg gcttcactat
      541 tttcacccag ttctgcttgc gctcactgtc aacagcaata taccttctcg agccttctgt
      601 tgggttaacc tgaagaagta atcccagcaa gtgtttccaa gatgtgcagg caacgattct
      661 gtaaagtact gaagcctcat tcaaacaatt accctgttat ccctagtcga ctagtatatg
      721 tgctgccgaa gcgagcacgg tgtttcgtcc tttccacaag atatataaag ccaagaaatc
      781 gaaatacttt caagttacgg taagcatatg atagtccatt ttaaaacata attttaaaac
      841 tgcaaactac ccaagaaatt attactttct acgtcacgta ttttgtacta atatctttgt
      901 gtttacagtc aaattaattc taattatctc tctaacagcc ttgtatcgta tatgcaaata
      961 tgaaggaatc atgggaaata ggccctcttc ctgcccgacc ctgg
//
