LOCUS       sequence-113675-         926 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA 
            epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Verzijlbergen KF, Menendez-Benito V, van Welsem T, van Deventer SJ, 
            Lindstrom DL, Ovaa H, Neefjes J, Gottschling DE, van Leeuwen F
  TITLE     Recombination-induced tag exchange to track old and new proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):64-8. doi: 
            10.1073/pnas.0911164107. Epub 2009 Dec 14.
  PUBMED    20018668
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0911164107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-01-5- 5"; volume: "107"; issue: "1"; pages: "64-8"
COMMENT     Sequence Label: pTW081
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    59..92
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             101..133
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     terminator      154..341
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(209..233)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(460..657)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 atagggcgaa ttgggcccaa tgcattggcg ccgcggccgc ggtggatctg gtggatctat
       61 aacttcgtat aatgtatgct atacgaagtt atcaggtacc atggcaagca tgactggtgg
      121 acagcaaatg ggtactagtt aacttctaaa taagcgaatt tcttatgatt tatgattttt
      181 attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt
      241 ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag
      301 gtatagtatg aggtcgctct tattgaccac acctctaccg gcagatccgc tagggataac
      361 agggtaagga tccgcattgt actgagagtg caccataatc atcgatgaat tcgagctcgt
      421 tttcgacact ggatggcggc gttagtatcg aatcgacagc agtatagcga ccagcattca
      481 catacgattg acgcatgata ttactttctg cgcacttaac ttcgcatctg ggcagatgat
      541 gtcgaggcga aaaaaaatat aaatcacgct aacatttgat taaaatagaa caactacaat
      601 ataaaaaaac tatacaaatg acaagttctt gaaaacaaga atctttttat tgtcagtact
      661 gattattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
      721 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
      781 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
      841 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
      901 cggagccgcg gcgatcctgc aagctc
//
