LOCUS       sequence-113734-         917 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  bacterial expression of GST-tagged ATF6 (1-150).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Sela D, Chen L, Martin-Brown S, Washburn MP, Florens L, Conaway JW, 
            Conaway RC
  TITLE     Endoplasmic reticulum stress-responsive transcription factor 
            ATF6alpha directs recruitment of the Mediator of RNA polymerase II 
            transcription and multiple histone acetyltransferase complexes.
  JOURNAL   J Biol Chem. 2012 Jun 29;287(27):23035-45. doi: 
            10.1074/jbc.M112.369504. Epub 2012 May 10.
  PUBMED    22577136
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.369504"; journalName: "J Biol Chem"; date: 
            "2012-06-29- 29"; volume: "287"; issue: "27"; pages: "23035-45"
COMMENT     Sequence Label: pET41s-GST-ATF6a-AD
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..47)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(519..542)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(581..603)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gcagcctagg tattaatcaa ttagtggtgg tggtggtggt ggtggtgctc gacggatccc
       61 tattccttca gtggctccgg tgaagagaga ctattagagt tttcaccata taaggaaagg
      121 ggagacatct gagaactaga agacaaatcc aactcctcag gaacatgctg agttgaagaa
      181 taagagtcca ctgaccgagg agatgagact gaataacttg aggaggctgg agaaagtggc
      241 tggggttctg ccttaatatc tttaactgta cagatttggt tgttgatgtc ccaaatgtct
      301 gactcccaag gcatcaaatc caaatcaaaa tcaagattat caaaattgtt ttcatacgtc
      361 tcatttgctg cttccaattg cagctcatca gtgtctgtga aataaccaag ttcagcaaag
      421 agagcagaat cccaatcttc atccagcctg tgaaagagtc ccgggctaaa aggtgactcc
      481 atggtgccgg caaccccagc cggctccccc atctcgaggg gcccctggaa cagaacttcc
      541 agactagttg aaccatccga ttttggagga tggtcgccac caccaaacgt ggcttgccag
      601 ccctgcaaag gccatgctat atacttgctg gatttcaagt acttatcaat ttgtgggata
      661 gcttcaatac gttttttaaa acaaactaat tttgggaacg catccaggca cattgggtcc
      721 atgtataaaa caacatcaag agcgtcatac aacatgaagt caggatgggt tacatgatca
      781 ccatttaaat atgttttatg acataaacga tcttcgaaca ttttcagcat ttcaggtagc
      841 ttgctaagaa aatcaacttt gagagtttca aagtctttac tatatgcaat tctcgaaaca
      901 ccgtatctaa tatccaa
//
