LOCUS       sequence-11374-e        4467 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L.
  TITLE     The Q System: A Repressible Binary System for Transgene Expression, 
            Lineage Tracing, and Mosaic Analysis
  JOURNAL   Volume 141, Issue 3, 536-548, 30 April 2010
  PUBMED    20434990
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Volume 141,
            Issue 3, 536-548, 30 April 2010"
COMMENT     Sequence Label: pUAS-GG
FEATURES             Location/Qualifiers
     source          1..4467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(33..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(143..164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     355..374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    679..773
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        792..1030
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     977..998
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     regulatory      1069..1078
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1120..1141)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(1366..1399)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(1551..1570)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1898..1919
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2092..2213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2129..2148)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2183..2202
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(2282..2300)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2321..2337)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2321..2337)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2334..2356)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2345..2361
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2369..2375,2376..2393,2394..2399))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2414..2435
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2552..2569)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2723..3311)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2803..2822)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3482..4273,4274..4342))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4105..4124
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4343..4447)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggatccaa
      661 gcttgcatgc ctgcaggtcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
      721 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagac
      781 tctagcgagc gccggagtat aaatagaggc gcttcgtcta cggagcgaca attcaattca
      841 aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagctgaa
      901 caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca
      961 accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg aatacaagaa
     1021 gagaactctg aatagggaat tgggaattcg ttaacagatc cgatatccgc caccatggtg
     1081 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1141 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1201 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1261 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1321 gacttcttca agtccgccat gcccgaaggt tggtatcaaa gatctataac ttcgtatagc
     1381 atacattata cgaagttatg gttacaagac aggtttaagg agaccaatag aaactgggca
     1441 tgtggagaca gagaagactc ttgggtttct gataggcact gactctctct gcctattggt
     1501 ctattttccc acccttaggg tacgtccagg agcgcaccat cttcttcaag gacgacggca
     1561 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1621 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1681 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggcgaact
     1741 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga
     1801 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     1861 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1921 ccgccgccgg gatcactctc ggcatggacg agctttacaa ggatatccct gaattctaga
     1981 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     2041 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     2101 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     2161 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatttaaat
     2221 tgggcgcgcc caactagttc tagagcggcc gcccaccgcg gtggagctcc agcttttgtt
     2281 ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2341 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2401 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2461 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2521 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2581 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2641 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2701 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2761 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2821 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2881 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2941 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3001 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3061 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3121 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     3181 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3241 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3301 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3361 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3421 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3481 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3541 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3601 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3661 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3721 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3781 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3841 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3901 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3961 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4021 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4081 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4141 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4201 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4261 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4321 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4381 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4441 ttccgcgcac atttccccga aaagtgc
//
