LOCUS       sequence-113896-         704 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  ZeoR mini-Tn7 vector, mobilizable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Zeo
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    177..224
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        335..382
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 tgtagcgtcg taagctaata cgaaaattaa aaatgacaaa atagtttgga actagatttc
       61 acttatctgg ttggcctgca aggccttcgc gaggtaccgg gcccaagctt ctcgaggaat
      121 tcctgcagcc cgggggatcc actagtgagc tcatgcatga tcgaattggg gatcttgaag
      181 tacctattcc gaagttccta ttctctagaa agtataggaa cttcagagcg cttttgaagc
      241 tgatgtgctt aaaaacttac tcaatggaat aattctagat attcggcttc gacgcgtaac
      301 tcacgttaag ggattttggt catgatcagc acgtgttgac aattaatcat cggcatagta
      361 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
      421 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
      481 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
      541 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
      601 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
      661 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgt
//
