LOCUS       sequence-113898-         694 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  ZeoR mini-Tn7 vector, mobilizable, for YFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Zeo-eyfp
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     248..266
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    312..328
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    345..361
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             380..391
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(443..464)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg acaaaatagt ttggaactag
       61 atttcactta tctggttggc ctgcaaggcc ttcgcgaggt accatttatc agggttattg
      121 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
      181 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
      241 ctataaaaat aggcgtatca cgaggccctt tcgtcttcac ctcgagaaaa tttatcaaaa
      301 agagtgttga cttgtgagcg gataacaatg atacttagat tcaattgtga gcggataaca
      361 atttcacaca tctagaatta aagaggagaa attaagcatg ctgagcaagg gcgaggagct
      421 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      481 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      541 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
      601 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      661 catgcccgaa ggctacgtcc aggagcgcac catc
//
