LOCUS       sequence-114018-        1395 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Chloride-conducting Channelrhodopsin with neuron-specific promoter, 
            plus red fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Wietek J, Beltramo R, Scanziani M, Hegemann P, Oertner TG, Simon 
            Wiegert J
  TITLE     An improved chloride-conducting channelrhodopsin for light-induced 
            inhibition of neuronal activity in vivo.
  JOURNAL   Sci Rep. 2015 Oct 7;5:14807. doi: 10.1038/srep14807.
  PUBMED    26443033
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-7"; volume: "5:14807. doi"; pages: " 
            10.1038/srep14807"
COMMENT     Sequence Label: pAAV CaMKIIa iChloC 2A tDimer
FEATURES             Location/Qualifiers
     source          1..1395
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..678
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     CDS             721..1395
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
ORIGIN
        1 atggtggcct cctccgagga cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
       61 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      181 atcctgtccc cccagttcca gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
      241 cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      301 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
      361 tacaaggtga agttccgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
      421 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
      481 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
      541 atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga caccaagctg
      601 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
      661 cgccaccacc tgttcctggg gcatggcacc ggcagcaccg gcagcggcag ctccggcacc
      721 gcctcctccg aggacgtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
      781 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      841 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
      901 tccccccagt tccagtacgg ctccaaggcg tacgtgaagc accccgccga catccccgac
      961 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1021 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     1081 gtgaagttcc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     1141 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     1201 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     1261 atggccaaga agcccgtgca gctgcccggc tactactacg tggacaccaa gctggacatc
     1321 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     1381 cacctgttcc tgtag
//
