LOCUS       sequence-114068-         800 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  OmpA redesign hybrid with wild-type strands 1358.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_H1358
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     23..85
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             533..559
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             611..628
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             629..646
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      660..707
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttacttttag gagataaaac atatgaaaaa gaccgcaatc gccatcgcag tggcactggc
       61 tggtttcgct accgtagcgc aggccgcgcc gaacgacaac acctggtata ctggtgctaa
      121 actgggctgg tcccagtacc acgacactgg tttcatcaat aataatggcc cgacccacga
      181 gaaccagcag ggtatcggtg cctttgcagg catccaggtg aacccgtacg ttggctttga
      241 aatgggttac gactggctcg gtcgtatgcc gtacaaaggc agcgttgaaa acggtgcata
      301 caaggctcag ggctatcaag cgacgctgaa aatgggtgtc ccgatcaccg acgacctgga
      361 tatctatact cgtctgggtg gcatggtatg gcgtgcagat actaaatcca acgtttatgg
      421 taaaaaccac gataccggcg tttctttcgt catggcgctg ggtagcgaac atgcgatcac
      481 tccggagatc gctatgcgtc tggaaacgca acacaccaac aacatcggtg actacccata
      541 cgatgttcca gattacgcga ctcgtccgga taacggcatg ctgagcctgg gtgtttccta
      601 ccgtttcggt tgttgccctg gttgctgtca ccatcaccac catcactaaa agcttccccc
      661 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgccc ctgagacgcg
      721 tcaatcgagt tcgtacctaa gggcgacacc ccataattag cccgggcgaa aggcccagtc
      781 tttcgactga gcctttcgtt
//
