LOCUS       sequence-114131-        2259 bp    DNA     circular SYN 15-APR-2025
DEFINITION  MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated
            (right promoter, weak constitutive down regulated by LuxR, C0062 in 
            combination with homoserine lactone (HSL)) [E:R0063:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: R0063 (pLuxR-pR)_EB
FEATURES             Location/Qualifiers
     source          1..2259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    167..187
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(193..210)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(364..952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(444..463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1123..1914,1915..1983))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1746..1765
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1984..2088)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2156..2174
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2178..2221)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2224..2245
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gcttacctgt acgatcctac aggtgcttat gttaagtaat tgtattccca gcgatacaat
       61 agtgtgacaa aaatccaatt tattagaatc aaatgtcaat ccattaccgt tttaatgata
      121 tataacacgc aaaacttgcg acaaacaata ggtaatacta gagacctact agtagcggcc
      181 gctgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      241 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      361 gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      781 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      961 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1021 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1081 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     1141 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1201 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1261 gcgcgaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1321 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1381 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1441 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1501 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     1561 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     1621 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     1681 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     1741 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     1801 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     1861 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     1921 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     1981 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     2041 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     2101 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     2161 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
     2221 ctggaattcg cggccgcttc tagagactag tgggtctca
//
