LOCUS       sequence-114340-        9081 bp    DNA     circular SYN 14-APR-2025
DEFINITION  expression of channelrhodopsin-2-YFP, a light-gated cation channel 
            that can depolarize cells via sodium influx when blue light changes 
            its conformation, using a DIO-based TLoop EIAV vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Cetin A, Callaway EM
  TITLE     Optical control of retrogradely infected neurons using 
            drug-regulated ""TLoop"" lentiviral vectors.
  JOURNAL   J Neurophysiol. 2014 May;111(10):2150-9. doi: 10.1152/jn.00495.2013.
            Epub 2014 Feb 26.
  PUBMED    24572099
REFERENCE   2  (bases 1 to 9081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00495.2013"; journalName: "J Neurophysiol"; date: 
            "2014-05"; volume: "111"; issue: "10"; pages: "2150-9"
COMMENT     Sequence Label: pEIAV-DIO-TLoop-CHR2YFP
FEATURES             Location/Qualifiers
     source          1..9081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        146..525
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        526..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(1913..1931,1932..1954,1955..1973,1974..1996,1997..2015,
                     2016..2038,2039..2057,2058..2080,2081..2099,2100..2122,
                     2123..2141,2142..2164,2165..2183)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2216..2254
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          2409..2638
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     2621..2640
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     protein_bind    complement(2696..2729)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(2780..2813)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2969..3586)
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             complement(3605..3658)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(3662..4369,4370..4372,4373..4375))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(3701..3722)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4050..4069
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4309..4330
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4372..4381
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(4391..5317)
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     regulatory      5314..5323
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    5328..5361
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    5412..5445
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5518..6106
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5571..5591)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5989..6000)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      6503..6958
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6590..6609)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6800..6821
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7027..7046)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7146..7168
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7206..7224)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7292..7396
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7397..7465,7466..8257)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7615..7634)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8428..9016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8917..8936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acagatcttc aatattggcc attagccata ttattcattg gttatatagc ataaatcaat
       61 attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc
      121 tcatgtccaa tatgaccgcc atgttggcat tgattattga ctagttatta atagtaatca
      181 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      241 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      301 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      361 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac
      421 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt
      481 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      541 cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      601 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      661 aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgggcactc agattctgcg gtctgagtcc cttctctgct
      781 gggctgaaaa ggcctttgta ataaatataa ttctctactc agtccctgtc tctagtttgt
      841 ctgttcgaga tcctacagtt ggcgcccgaa cagggacctg agaggggcgc agaccctacc
      901 tgttgaacct ggctgatcgt aggatccccg ggacagcaga ggagaactta cagaagtctt
      961 ctggaggtgt tcctggccag aacacaggag gacaggtaag tagggagacc ctttgacatg
     1021 gagcaaggcg ctcaagaagt tagagaaggt gacggtacaa gggtctcaga aattaactac
     1081 tggtaactgt aattgggcgc taagtctagt agacttattt catgatacca actttgtaaa
     1141 agaaaaggac tggcagctga gggatgtcat tccattgctg gaagatgtaa ctcagacgct
     1201 ggaattcggg tattgtagaa agggcaaata ggaccttgaa agagaagatt caaagtcata
     1261 gagacaacac tcaaacactg gaggcagctt tacaacttgc tctcattact tgtaacaaag
     1321 ggagggaaag tatgggagga cagacaccat gggaagtatt tatcactaat caagcacaag
     1381 taatacatga gaaactttta ctacagcaag cacaatcctc caaaaaattt tgtttttaca
     1441 aaatccctgg tgaacatgat tggaagggac ctactagggt gctgtggaag ggtgatggtg
     1501 cagtagtagt taatgatgaa ggaaagggaa taattgctgt accattaacc aggactaagt
     1561 tactaataaa gccaaattga gtattgttgc atgcatttga tatatgggat tatttggtaa
     1621 aggggtaaca tggtcagcat cgcattctat ggggggatcc cagggggaat ctcaacccct
     1681 attacccaac agtcagaaaa atctaagtgt gaggagaaca caatgtttca accttattgt
     1741 tataataatg acagtaagaa cagcatggca gaatcgaagg aagcaagaga ccaagaaatg
     1801 aacctgaaag aagaatctaa agaagaaaaa agaagaaatg actggtggaa aaaagctgca
     1861 gagggccctt aatgcagtgg cctttgcggc cgccaactct cgagtttacc actccctatc
     1921 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     1981 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     2041 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     2101 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     2161 ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgaggtag
     2221 gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2281 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg
     2341 cggccccgaa ttcaccacgt ggccgcatcg attctagaat tcgctgtctg cgagggccag
     2401 ctgttggggt gagtactccc tctcaaaagc gggcatgact tctgcgctaa gattgtcagt
     2461 ttccaaaaac gaggaggatt tgatattcac ctggcccgcg gtgatgcctt tgagggtggc
     2521 cgcgtccatc tggtcagaaa agacaatctt tttgttgtca agcttgaggt gtggcaggct
     2581 tgagatctgg ccatacactt gagtgacaat gacatccact ttgcctttct ctccacaggt
     2641 gtccactccc aggtccaact gcagcccaag cggatcctct agagtcgact ccggaataac
     2701 ttcgtatagg atactttata cgaagttatg cagaatggta gctggattgt agctgctatt
     2761 agcaatatga aacctcttaa taacttcgta tagcatacat tatacgaagt tatggcgcgc
     2821 ctcacagcat atccaggtca aaatcatcaa gtgcatcagc agggagcatg tccaaatcaa
     2881 agtcatccag ggcatcagca ggcagcatgt ccaggtcaaa atcatccaag gcatcagcag
     2941 gcacgtgctt tctctttttg ggcacgtgag agccagactc acatttcagc tgcttttcca
     3001 ggccacagat gatcagttcc aggccaaaaa ggaatgcagg ctcagcccct tggtggtcaa
     3061 acaactcaat agcttgccgc agcagtggtg gcatggagtc agtggtgggg gtctccctct
     3121 cctccttggc aacttggtgc tcctggtcct ccagcacaca gcccaaggtg aagtggccca
     3181 cagcagagag ggcatacaag gcattttcca gggagaagcc ttgttggcac aggaatgcca
     3241 gctggttctc cagggtctca tactgcttct ctgtaggcct ggtgcccaag tgaactttgg
     3301 caccatcccg gtgggagagc aaggcacatc tgaagctctt ggcattgttc ctcaggaagt
     3361 cctgccagga ctccccttcc agagggcaga agtgggtgtg gtgcctgtcc aacatctcaa
     3421 tggccagggc atcaagcaga gccctcttgt tcttcacatg ccagtacagg gtaggctgct
     3481 ccacacccag cttctgggcc agcttcctgg tggtcaagcc ctcaatgcca acttcattca
     3541 ggagctccaa ggcagagttg atgactttgc tcttgtccag tctggaggta ccgctgccgg
     3601 gggcggggcc ggggttctcc tccacgtcgc cgcaggtcag caggctgccg cggccctccg
     3661 cgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     3721 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctggtagctc aggtagtggt
     3781 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     3841 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
     3901 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
     3961 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     4021 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     4081 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     4141 tgtggtcggg gtagcgggcg aagcactgca ggccgtagcc gaaggtggtc acgagggtgg
     4201 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     4261 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     4321 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg
     4381 cggccgctgg cacggctccg gcctcggctt cgtcttcgac gagagtctcg acctcgatct
     4441 ccgttccgcc gatgttcagt ttggtggttt tgcggatatc tccgtgaatc aatatgtgct
     4501 cgtggatcag gacgcgcagg tagtgtccca acaaccccca acaattttta ctcatcagat
     4561 caataatcgt gtgacctacg gtggagccat agacgctcag gacgccaaaa ccttcgggcc
     4621 ccaaaatgaa gagaattggg aacatacccc agctcacgaa aaacagccat gccatgccgg
     4681 tcacgacctg gcggcaccga ccctttggca cagtatgata accctcgata tatgctttgg
     4741 cggcgtgaaa aaatgtgttc gcgccatagc acaatccaag acaaaagaag atgactttaa
     4801 catagccggt tgccatggcg ctggtagccc cccacacgat agtcccgatg tctgagacaa
     4861 ggagtcccat ggttctcctg ctgtagtcgt tgctcaggcc ggtgaggttg ctcaggcgga
     4921 taaggatgac aggacaagtg agcagccact ctgcatagcg cagccactgc acccggtgtc
     4981 ctgtggcaag gtagagcata gagggattct taaactcaaa aaagaactcg agaatcacct
     5041 taaccatttc aatggcgcac acatagatct cctcccagcc gcatgtagat ttccaggttt
     5101 ggtaggcata gaacatcagc agcaaaatgc tgaatcctgc tgcaagccac tgcaggacat
     5161 ttgacgcggt ctgagcgccg ttcgtgccgc gagattcaat ccatccggca cagtaacatt
     5221 gatcctcagg gaccaggacg gacccgttca ccaccacagg attagtaacg aacaaaagtt
     5281 cgcgtccgac ggcagacaaa gcgccgccat agtccatggt ggctagcata acttcgtata
     5341 aagtatccta tacgaagtta tttgccttaa cccagaaatt atcactgtta ttctttagaa
     5401 tggtgcaaag aataacttcg tataatgtat gctatacgaa gttatgaatt cgatatcaag
     5461 cttatcgata atcaacatgt taagaaaatt tcccgttatt tgcactctgt tcctgttaat
     5521 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5581 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5641 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5701 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5761 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5821 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5881 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     5941 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6001 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6061 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgt ttcgcctcgg
     6121 cgtccggtcc gtgttgcttg gtcttcacct gtgcagactt gcgaaccatg gattccaccg
     6181 tgaactttgt ctcctggcat gcaaatcgtc aacttggcat gccaaatcga ttagaaaaac
     6241 aaggggggaa ctgtggggtt tttatgaggg gttttataca attgggcact cagattctgc
     6301 ggtctgagtc ccttctctgc tgggctgaaa aggcctttgt aataaatata attctctact
     6361 cagtccctgt ctctagtttg tctgttcgag atcctacaga gctcatgcct tggttaatta
     6421 aggagatccg ggctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     6481 gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6541 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6601 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6661 ctccctttag ggttccgatt tagagcttta cggcacctcg accgcaaaaa acttgatttg
     6721 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6781 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6841 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6901 gagctgattt aacaaatatt taacgcgaat tttaacaaaa tattaacgtt tacaatttcg
     6961 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     7021 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     7081 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     7141 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     7201 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     7261 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7321 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7381 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7441 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7501 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7561 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7621 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7681 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7741 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7801 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7861 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7921 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7981 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8041 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8101 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8161 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8221 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8281 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8341 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8401 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8461 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8521 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     8581 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8641 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8701 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8761 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8821 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8881 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8941 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9001 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9061 gccttttgct cacatggctc g
//
