LOCUS       sequence-114404-         959 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Cloning, T7 RNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Salat-Canela C, Sese M, Peula C, Ramon y Cajal S, Aasen T
  TITLE     Internal translation of the connexin 43 transcript.
  JOURNAL   Cell Commun Signal. 2014 May 8;12:31. doi: 10.1186/1478-811X-12-31.
  PUBMED    24884945
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Commun
            Signal."; date: "2014-05-8"; volume: "12:31. doi"; pages: " 
            10.1186/1478-811X-12-31"
COMMENT     Sequence Label: pBS-Cx43
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    133..154
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(169..174,175..192,193..199)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    207..223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     212..234
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     231..247
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     231..247
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     266..286
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        268..286
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     323..339
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     323..339
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    366..390
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
       61 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      121 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      181 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
      241 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
      301 gctccaccgc ggtggcggcc gctctagaac tagtggatcc cccgggctgc aggaattcga
      361 tatcaaccac tttgtacaag aaagctgggt actagatctc caggtcatca ggccgaggtc
      421 tgctgctggc acgactgctg gctctgcttg aaggtcgctg gtccacaatg gctagtggct
      481 gtaattcatg tccagcagct agtttttttg aattctggtt atcatcgggg aaatcaaaag
      541 gctgtgcatg ggagttagag atggtgcttc ccgcctgccc cattcgattt tgttctgcac
      601 tgtaattagc ccagttttgc tcacttgctt gcttgttgta attgcggcaa gaagaattgt
      661 ttctgtcgcc agtaaccagc ttgtacccag gaggagacat aggcgagagg ggagcggttg
      721 gtgaggagca gccattgaaa taagcatatt tttgagaccc acagtctttg gcagggctca
      781 gcgcaccact ggtcgcatgg taagggtcgc tctttccctt aacccgatcc ttaacgccct
      841 tgaagaaaac atagaagagt tcaatgatat tcaaggccag ggacaccaag gacaccacca
      901 gcatgaagat gatgaagatg gttttctccg tggggcgaga gaggaaacag tccacctga
//
