LOCUS       sequence-114416-         954 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Cloning, T7 RNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Salat-Canela C, Sese M, Peula C, Ramon y Cajal S, Aasen T
  TITLE     Internal translation of the connexin 43 transcript.
  JOURNAL   Cell Commun Signal. 2014 May 8;12:31. doi: 10.1186/1478-811X-12-31.
  PUBMED    24884945
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Commun
            Signal."; date: "2014-05-8"; volume: "12:31. doi"; pages: " 
            10.1186/1478-811X-12-31"
COMMENT     Sequence Label: pBS-Cx43-FS
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     161..183
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     175..192
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     176..192
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     202..221
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        202..220
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     245..261
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     246..262
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    278..302
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
       61 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
      121 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
      181 acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg taccgggccc
      241 cccctcgagg tcgacggtat cgataagctt gatatcaaca agtttgtaca aaaaagcagg
      301 cttcatgggt gactggagcg ccttaggcaa actccttgca aggttcaagc ctactcaact
      361 gctggaggga aggtgtggct gtcagtactt ttcattttcc gaatcctgct gctggggaca
      421 gcggttgagt cagcctgggg agatgagcag tctgcctttc gttgtaacac tcagcaacct
      481 ggttgtgaaa atgtctgcta tgacaagtct ttcccaatct ctcatgtgcg cttctgggtc
      541 ctgcagatca tatttgtgtc tgtacccaca ctcttgtacc tggctcatgt gttctatgtg
      601 atgcgaaagg aagagaaact gaacaagaaa gaggaagaac tcaaggttgc ccaaactgat
      661 ggtgtcaatg tggacatgca cttgaagcag attgagataa agaagttcaa gtacggtatt
      721 gaagagcatg gtaaggtgaa aatgcgaggg gggttgctgc gaacctacat catcagtatc
      781 ctcttcaagt ctatctttga ggtggccttc ttgctgatcc agtggtacat ctatggattc
      841 agcttgagtg ctgtttacac ttgcaaaaga gatccctgcc cacatcaggt ggactgtttc
      901 ctctctcgcc ccacggagaa aaccatcttc atcatcttca tgctggtggt gtcc
//
