LOCUS       sequence-114474-         603 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Thai TH, Calado DP, Casola S, Ansel KM, Xiao C, Xue Y, Murphy A, 
            Frendewey D, Valenzuela D, Kutok JL, Schmidt-Supprian M, Rajewsky N,
            Yancopoulos G, Rao A, Rajewsky K
  TITLE     Regulation of the germinal center response by microRNA-155.
  JOURNAL   Science. 2007 Apr 27. 316(5824):604-8.
  PUBMED    17463289
REFERENCE   2  (bases 1 to 603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2007 Apr 27. 316(5824):604-8."
COMMENT     Sequence Label: CTV
FEATURES             Location/Qualifiers
     source          1..603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    66..114
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    121..154
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(196..215)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(277..299)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
       61 gggatcggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcggatcc
      121 ataacttcgt ataatgtatg ctatacgaag ttatttaatt aactcgagga attctttgcc
      181 aaaatgatga gacagcacaa taaccagcac gttgcccagg agctgtagga aaaagaagaa
      241 ggcatgaaca tggttagcag aggctctaga gccgccggtc acacgccaga agccgaaccc
      301 cgccctgccc cgtccccccc gaaggcagcc gtccccccgc ggacagcccc gaggctggag
      361 agggagaagg ggacggcggc gcggcgacgc acgaaggccc tccccgccca tttccttcct
      421 gccggcgccg caccgcttcg ccccgcgccc gctagagggg gtgcggcggc gcctcccaga
      481 tttcggctcc gcacagattt gggacaaagg aagtccctgc gccctctcgc acgattacca
      541 taaaaggcaa tggctgcggc tcgccgcgcc tcgacagccg ccggcgctcc gggggccgcc
      601 gcg
//
