LOCUS       sequence-114601-         820 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Actin Bundling Protein, Excitation: 448 / 473, 
            Emission: 500 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: miniSOG-Fascin-C-10
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..351
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     polyA_signal    590..711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(627..646)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     681..700
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ccgtcagatc cgctagcgct accggtcgcc accatggaga aaagtttcgt gataactgat
       61 ccacggctgc cagacaatcc catcatcttc gcatccgatg gcttcctgga gctgaccgag
      121 tattccagag aggagatcct gggccgcaat ggccgctttc tgcagggacc agagacagac
      181 caggccacag tgcagaagat tcgcgatgcc attagagatc agcgcgagat taccgtgcag
      241 ctgataaact acacaaaaag cgggaagaaa ttctggaacc tcctgcacct ccagcccatg
      301 agggaccaga agggtgagct ccagtatttc atcggagtgc agctggatgg atccggactc
      361 agatctaagc tgaaccctcc tgatgagagt ggccccggct gcatgagctg caagtgtgtg
      421 ctctcctgag aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata
      481 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
      541 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
      601 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
      661 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa
      721 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
      781 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
//
