LOCUS       sequence-114662-        1039 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  pETDuet-1 Containing FadD G446S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS510 (TJF032 G446S)
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    144..168
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(168..187)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(169..187)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(276..293)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgctggcgtt caaatttcgc agcagcggtt tctttaccag actcgagggt accgacgtca
       61 gcgatcgcgt ggccggccga tatccaattg agatctgcca tatgtatatc tccttcttat
      121 acttaactaa tatactaaga tggggaattg ttatccgctc acaattcccc tatagtgagt
      181 cgtattaatt tcgattatgc ggccgtgtac aatacgatta ctttctgttc gacttaagca
      241 ttatgcggcc gcaagcttgg atcctcattc attcagtgat gatgatggtg atgagaagaa
      301 cctcaggctt tattgtccac tttgccgcgc gcttcgtcac gtaattctcg tcgcaaaatt
      361 tttccgacgt tagatttcgg taactcatca cgaaactcca ccagcttcgg tactttgtag
      421 cccgtgagct gacggcggca aaaggtcacc agtgactctt cggtaagcga tggatctttt
      481 ttcactacga agattttcac cgcttcacca ctggagccgg aaggtacgcc aacagccgcg
      541 acttcctgta cgccaggatg ctgcatgacg acatcttcaa tctcgttggg atagacgtta
      601 aaaccggaaa ccagaatcat gtctttttta cgatcgacaa tgcgcaggaa agattcttca
      661 tccatcaccg cgatgtcgcc ggtgtgtaac cagccatttt tgatgatctc atctgtagca
      721 tccggacgct gccagtaacc cagcatcacc tgcggtcctt tgacacaaag ctcacccggt
      781 tgacccggtg gtacttcatt atcatcatca tccaccagtt tggcttccgt cgacggcacc
      841 ggcaaaccga tgctaccact atgataatca atatcatatg ggttaacgct gaccagcggc
      901 gcacactcgg taaggccata gccttccagc agatactgtc ctgtcagttt cacccaacgc
      961 tctgccacca cttgctgcac tggcatccct ccgcctgcgg aaagatgcag actggaaaaa
     1021 tccagctgct ggaactctt
//
