LOCUS       sequence-114689-        1034 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Plasmid for tet-inducible expression of dCas9-EGFP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ochiai H, Sugawara T, Yamamoto T
  TITLE     Simultaneous live imaging of the transcription and nuclear position 
            of specific genes.
  JOURNAL   Nucleic Acids Res. 2015 Jun 19. pii: gkv624.
  PUBMED    26092696
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jun 19. pii: gkv624."
COMMENT     Sequence Label: pBSK-Delta-B-TRE-dCas9-EGFP
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..56
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             60..80
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagctcggta cccgaccggt gccaccatgt acccatacga tgttccagat tacgcttcgc
       61 cgaagaaaaa gcgcaaggtc gaagcgtccg acaagaagta cagcatcggc ctggccatcg
      121 gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat
      181 tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc
      241 tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat
      301 acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca
      361 aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga
      421 agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt
      481 accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc
      541 ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg
      601 gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct
      661 acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc
      721 tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg
      781 agaagaagaa tggcctgttc ggcaacctga ttgccctgag cctgggcctg acccccaact
      841 tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg
      901 acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg
      961 ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga
     1021 tcaccaaggc cccc
//
