LOCUS       sequence-114884-        1065 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  pETDuet-1 Containing FadD Y371G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS527 (TJF094)
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..49)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    158..182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(182..201)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(183..201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(290..307)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagacgagtc catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta ccagactcga
       61 gggtaccgac gtcagcgatc gcgtggccgg ccgatatcca attgagatct gccatatgta
      121 tatctccttc ttatacttaa ctaatatact aagatgggga attgttatcc gctcacaatt
      181 cccctatagt gagtcgtatt aatttcgatt atgcggccgt gtacaatacg attactttct
      241 gttcgactta agcattatgc ggccgcaagc ttggatcctc attcattcag tgatgatgat
      301 ggtgatgaga agaacctcag gctttattgt ccactttgcc gcgcgcttcg tcacgtaatt
      361 ctcgtcgcaa aatttttccg acgttagatt tcggtaactc atcacgaaac tccaccagct
      421 tcggtacttt gtagcccgtg agctgacggc ggcaaaaggt caccagtgac tcttcggtaa
      481 gcgatggatc ttttttcact acgaagattt tcaccgcttc accactggag ccggaaggta
      541 cgccaacagc cgcgacttcc tgtacgccag gatgctgcat gacgacatct tcaatctcgt
      601 tgggatagac gttaaaaccg gaaaccagaa tcatgtcttt tttacgatcg acaatgcgca
      661 ggaacccttc ttcatccatc accgcgatgt cgccggtgtg taaccagcca tttttgatga
      721 tctcatctgt agcatccgga cgctgccagt aacccagcat cacctgcggt cctttgacac
      781 aaagctcacc cggttgaccc ggtggtactt cattatcatc atcatccacc agtttggctt
      841 ccgtcgacgg caccggcaaa ccgatgctac cactatgata atcaatatcg cctgggttaa
      901 cgctgaccag cggcgcacac tcggtaaggc catagccttc cagcagatac tgtcctgtca
      961 gtttcaccca acgctctgcc accacttgct gcactggcat ccctccgcct gcggaaagat
     1021 gcaaactgga aaaatccagc tgctggaact ctttattgtt cagca
//
