LOCUS       sequence-114927-         820 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  plasmid for bacterial overexpression of HuD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  TITLE     Identification of the transcriptome-wide mRNA targets of the 
            neuronal ELAVL proteins (unpublished)
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET23a_mod_HuD_FL
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..45
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             61..84
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             85..111
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggagatatac atatggctag catgactggt ggacagcaaa tgggtcgcgg atcgaccggt
       61 gactacaagg acgacgatga caagtaccct tatgacgtgc ccgattacgc tggatccgaa
      121 ttcgagctcc gtcgacccat ggttatgata attagcacca tggagcctca ggtgtcaaat
      181 ggtccgacat ccaatacaag caatggaccc tccagcaaca acagaaactg tccttctccc
      241 atgcaaacag gggcaaccac agatgacagc aaaaccaacc tcatcgtcaa ctatttaccc
      301 cagaatatga cccaagaaga attcaggagt ctcttcggga gcattggtga aatagaatcc
      361 tgcaaacttg tgagagacaa aattacagga cagagtttag ggtatggatt tgttaactat
      421 attgatccaa aggatgcaga gaaagccatc aacactttaa atggactcag actccagacc
      481 aaaaccataa aggtctcata tgcccgtccg agctctgcct caatcaggga tgctaacctc
      541 tatgttagcg gccttcccaa aaccatgacc cagaaggaac tggagcaact tttctcgcaa
      601 tacggccgta tcatcacctc acgaatcctg gttgatcaag tcacaggagt gtccagaggg
      661 gtgggattca tccgctttga taagaggatt gaggcagaag aagccatcaa agggctgaat
      721 ggccagaagc ccagcggtgc tacggaaccg attactgtga agtttgccaa caaccccagc
      781 cagaagtcca gccaggccct gctctcccag ctctaccagt
//
