LOCUS       sequence-114932-         820 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  expresses GFP-tagged RBPMS2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pFRT-TODestGFP_RBPMS2
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     38..62
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(159..161,162..164,165..677)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(204..225)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(465..484)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga
       61 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      121 gcctccggac tctagcgttt aaacttaagc ttggtaccat ggtgagcaag ggcgaggagc
      181 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      241 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      301 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
      361 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      421 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      481 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      541 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
      601 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
      661 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
      721 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
      781 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
//
