LOCUS       sequence-114948-         721 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet28a_RBPMS_20_196
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(605..637)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(641..658)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(668..685)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtcagtgggg gtggtggtgg tgctcgagtg cggccgctca gcagaactga cgggacttcc
       61 agccctgaga agtagcctcg gagggaggga gccagcgcat ctgggcatgc agtgaagcgg
      121 gataggtgaa agcaggagga ggtagagcag gcgctaactc cgctgggtac agagggtacg
      181 gggcccacac ttcagggcta ctggggtaaa gtgcaggcac tgtgagctca tatggctctc
      241 tggcaatgaa ctgaggtaca gtgttgggca gaggagtact ggggtttgga gtccctacga
      301 gtttgttctt ggccatcttc gtgtttgcct tagcaaactc tagtcgtagt gtttgcggaa
      361 tttcaggatc gaagcggatg ccattcaaag cattctttgc agcctctgct tctgagcgac
      421 tgtcaaaact gacaaaacct acaggctgtt tagatgtgag ctttataaga gaaccctcat
      481 agcccttaaa tggtctgaaa agcagataga gctcccgagg tttgatatcc agaggaaggc
      541 cactgacaaa tagggtccgg acctcctcct ccatgggtcg acggagctcg aattcggatc
      601 cgcgacccat ttgctgtcca ccagtcatgc tagccatatg gctgccgcgc ggcaccaggc
      661 cgctgctgtg atgatgatga tgatggctgc tgcccatggt atatctcctt cttaaagtta
      721 a
//
