LOCUS       sequence-11496-e        1851 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Schowalter RM, Pastrana DV, Pumphrey KA, Moyer AL, Buck CB.
  TITLE     Merkel cell polyomavirus and two previously unknown polyomaviruses 
            are chronically shed from human skin.
  JOURNAL   Cell Host Microbe. 2010 Jun 25;7(6):509-15.
  PUBMED    20542254
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2010-06-25"; volume: "7(6)"; pages: "509-15"
COMMENT     Sequence Label: pAsylum
FEATURES             Location/Qualifiers
     source          1..1851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(219..305)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     458..475
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     rep_origin      683..1271
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1172..1191
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1357..1404
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        1396..1414
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccctgcaggg cctgaaataa cctctgaaag aggaacttgg ttaggtacgc aactgttggg
       61 aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc cggatcctta tcgattttac
      121 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      181 acataaaatg aatgcaattg ttgttgttct cccggcggat ttgtcctact caggagagcg
      241 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
      301 atttgatgcc tggcagttcc ctactctcgc atggggagac cccacactac catcggcgct
      361 acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc tactgccgcc
      421 aggcaaattc tgttttatca gaccgcttct gcgttctgat ttaatctgta tcaggctgaa
      481 aatcttctct catccgccaa aacagccaag cttcatgttg ctaaaccaat gttaagtgaa
      541 tctttggaaa caaaatgttt ccaaattact gggatgtgca tgttgaaacg tgggttaatt
      601 aactagccat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
      661 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
      721 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      781 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      841 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      901 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      961 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1021 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1081 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1141 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1201 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1261 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1321 ttgctcacat gttcttaatt aaatttttca aaagtagttg acaattaatc atcggcatag
     1381 tatatcggca tagtataata cgactcacta taggagggcc atcatggcca agttgaccag
     1441 tgctgtccca gtgctcacag ccagggatgt ggctggagct gttgagttct ggactgacag
     1501 gttggggttc tccagagatt ttgtggagga tgactttgca ggtgtggtca gagatgatgt
     1561 caccctgttc atctcagcag tccaggacca ggtggtgcct gacaacaccc tggcttgggt
     1621 gtgggtgaga ggactggatg agctgtatgc tgagtggagt gaggtggtct ccaccaactt
     1681 cagggatgcc agtggccctg ccatgacaga gattggagag cagccctggg ggagagagtt
     1741 tgccctgaga gacccagcag gcaactgtgt gcactttgtg gcagaggagc aggactgagg
     1801 ataagaattg taacaaaaaa ccccgccccg gcggggtttt ttgttaatta a
//
