LOCUS       sequence-115060-         829 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Destination vector with RBPMS resistant to siRNA treatment by s21729
            (Applied Biosystems) with K100E mutation for stable cell line 
            generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_KDR_K100E
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..37
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     47..71
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             171..194
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             195..221
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    237..261
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga
       61 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
      121 accgatccag cctccggact ctagcgttta aacttaagct tggtaccatg gactacaagg
      181 acgacgatga caagtaccct tatgacgtgc ccgattacgc tttgaaccag atatcaacaa
      241 gtttgtacaa aaaagcaggc tccaccatgg gaaccaattc agtcgacatg aacaacggcg
      301 gcaaagccga gaaggagaac accccgagcg aggccaacct tcaggaggag gaggtccgga
      361 ccctatttgt cagtggcctt cctctggaca ttaaacctcg ggagctctat ctgcttttca
      421 gaccatttaa gggctatgag ggttctctta taaagctcac atctaaacag cctgtaggtt
      481 ttgtcagttt tgacagtcgc tcagaagcag aggctgcaaa gaatgctttg aatggcatcc
      541 gcttcgatcc tgaaattccg caaacactac gactagagtt tgctgaggca aacacgaaga
      601 tggccaagaa caaactcgta gggactccaa accccagtac tcctctgccc aacactgtac
      661 ctcagttcat tgccagagag ccatatgagc tcacagtgcc tgcactttac cccagtagcc
      721 ctgaagtgtg ggccccgtac cctctgtacc cagcggagtt agcgcctgct ctacctcctc
      781 ctgctttcac ctatcccgct tcactgcatg cccagatgcg ctggctccc
//
