LOCUS       sequence-115061-         780 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Destination vector with RBPMS resistant to siRNA treatment by s21729
            (Applied Biosystems) with K100E mutation for stable cell line 
            generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_KDR_K100E
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(44..85)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    138..162
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggcacactgg atcagttatc tatgcggccg cggatcaggt acgcgtagaa tcgagaccga
       61 ggagagggtt agggataggc ttaccttcga accgcgggcc ctctagactt gagcggccgc
      121 cactgtgctg gatatcaacc actttgtaca agaaagctgg gtctagatat ctcgagtgcg
      181 gccgctcagc agaactgacg ggacttccag ccctgagaag tagcctcgga gggagggagc
      241 cagcgcatct gggcatgcag tgaagcggga taggtgaaag caggaggagg tagagcaggc
      301 gctaactccg ctgggtacag agggtacggg gcccacactt cagggctact ggggtaaagt
      361 gcaggcactg tgagctcata tggctctctg gcaatgaact gaggtacagt gttgggcaga
      421 ggagtactgg ggtttggagt ccctacgagt ttgttcttgg ccatcttcgt gtttgcctca
      481 gcaaactcta gtcgtagtgt ttgcggaatt tcaggatcga agcggatgcc attcaaagca
      541 ttctttgcag cctctgcttc tgagcgactg tcaaaactga caaaacctac aggctgttta
      601 gatgtgagct ttataagaga accctcatag cccttaaatg gtctgaaaag cagatagagc
      661 tcccgaggtt taatgtccag aggaaggcca ctgacaaata gggtccggac ctcctcctcc
      721 tgaaggttgg cctcgctcgg ggtgttctcc ttctcggctt tgccgccgtt gttcatgtcg
//
