LOCUS       sequence-115569-         768 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  synchronize trafficking of ST from the ER (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: Ii-Str_ST-SBP-mCherry
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             290..403
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     primer_bind     complement(558..576)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
       61 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
      121 gataagcttg ccacaacccg ggaggcgcgc catgattcac accaacctga agaaaaagtt
      181 cagctgctgc gtcctggtct ttcttctgtt tgcagtcatc tgtgtgtgga aggaaaagaa
      241 gaaagggagt tactatgatt cctttaaatt gcaaaccaag gggaattcca tggacgagaa
      301 gaccactggt tggcgaggtg gacacgttgt tgaaggactg gctggggaac ttgaacaact
      361 tcgtgcacga ctggagcatc acccacaagg tcaacgtgaa ccacctgcag gtatggtgag
      421 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
      481 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
      541 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
      601 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
      661 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
      721 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctaca
//
