LOCUS       sequence-115576-         744 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  synchronize trafficking of Ecadherin from the ER (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: Ii-Str_SBP-EGFP-Ecadherin
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     152..211
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             212..322
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     primer_bind     complement(377..398)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(638..657)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
       61 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
      121 gataagcttg ccacaacccg ggaggcgcgc catgtacagg atgcaactcc tgtcttgcat
      181 tgcactaagt cttgcacttg tcacgaattc cgacgagaag accactggtt ggcgaggtgg
      241 acacgttgtt gaaggactgg ctggggaact tgaacaactt cgtgcacgac tggagcatca
      301 cccacaaggt caacgtgaac cacctgcagg tatggtgagc aagggcgagg agctgttcac
      361 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      421 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      481 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
      541 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      601 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      661 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      721 cttcaaggag gacggcaaca tcct
//
