LOCUS       sequence-115588-         934 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Thai TH, Calado DP, Casola S, Ansel KM, Xiao C, Xue Y, Murphy A, 
            Frendewey D, Valenzuela D, Kutok JL, Schmidt-Supprian M, Rajewsky N,
            Yancopoulos G, Rao A, Rajewsky K
  TITLE     Regulation of the germinal center response by microRNA-155.
  JOURNAL   Science. 2007 Apr 27. 316(5824):604-8.
  PUBMED    17463289
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2007 Apr 27. 316(5824):604-8."
COMMENT     Sequence Label: CTV
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    371..505
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(408..427)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     462..481
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(525..558)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    591..638
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(820..837)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
       61 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
      121 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
      181 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
      241 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
      301 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacatagaa tgaatgcaat
      361 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      421 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      481 caatgtatct tatcatgtct ggatctgaca tggtaagtaa gcttataact tcgtatagca
      541 tacattatac gaagttatcg gcgcgccgcg gccgcgatca attcggtacc gaagttccta
      601 ttccgaagtt cctattctct agaaagtata ggaacttcct cgagggtacc ccaattccgc
      661 cccccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
      721 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
      781 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
      841 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
      901 ctgtagcgac cctttgcagg cagcggaacc cccc
//
