LOCUS       sequence-115716-         659 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Vector for Cas9n (D10A) mRNA synthesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Ohtsuka M
  TITLE     Ohtsuka lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBHD
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             138..164
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             168..188
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt
      121 gcaccggtgc caccatgtac ccatacgatg ttccagatta cgcttcgccg aagaaaaagc
      181 gcaaggtcga agcgtccgac aagaagtaca gcatcggcct ggccatcggc accaactctg
      241 tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg
      301 gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg
      361 gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga
      421 agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca
      481 gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc
      541 accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct
      601 accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctat
//
