LOCUS       sequence-115898-        6087 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Retroviral expression of mouse c-Myc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Takahashi K, Yamanaka S
  TITLE     Induction of pluripotent stem cells from mouse embryonic and adult 
            fibroblast cultures by defined factors.
  JOURNAL   Cell. 2006 Aug 25. 126(4):663-76.
  PUBMED    16904174
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Aug 25. 126(4):663-76."
COMMENT     Sequence Label: pMXs-c-Myc
FEATURES             Location/Qualifiers
     source          1..6087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             120..589
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     protein_bind    906..930
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2287..2311)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2362..2736
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(2408..2424)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(2746..3162)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(2762..2778)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2794..2816)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    3221..3578
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             3642..4158
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     promoter        4189..4293
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4294..4362,4363..5154)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4512..4531)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5318..5906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5807..5826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6060..6077
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcgtcgacc actgtgctgg cggccgctcg
      781 agtttaaata gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg
      841 gttattatta tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcagctgga
      901 atatcaccac tttgtacaag aaagctgggt cggcgcgccc acccttttat gcaccagagt
      961 ttcgaagctg ttcgagtttg tgtttcaact gttctcgtcg tttcctcaat aagtcctttt
     1021 cagaggtgag cttgtgctcg tctgcttgaa tggacaggat gtaggcggtg gcttttttga
     1081 ggatcactac cttgggggcc ttttcgttgt tttccaattc agggatctgg tcacgcaggg
     1141 caaaaaagct gcgcttcagc tcgttcctcc tctgacgttc caagacgttg tgtgtccgcc
     1201 tcttgtcgtt ttcctccgtg tctgaggacc tggggctgga gcacttgcgg ttgttgctga
     1261 tctgcttcag gaccctgcca ctgtccaact tggccctctt ggcagctgga tagtccttcc
     1321 ttgtggaggg gggtgcggcg tagttgtgct ggtgagtgga gacgtggcac ctcttgagga
     1381 ccagtgggct gtgcggaggt ttgctgtggc ctcgggatgg agatgagccc gactccgacc
     1441 tcttggcagg ggtttgcctc ttctccacag acaccacatc aatttcttcc tcatcttctt
     1501 gctcttcttc agagtcgctg ctggtggtgg gcggtgtctc ctcatgcagc actaggggct
     1561 cagggctggc ccgtggggag gactcggagg acagcagcga gtccgaggaa ggagagaagg
     1621 ccgtggaatc ggacgaggta caggatttgg gcgagctgct gtcgttgagc gggtagggaa
     1681 agaccactga ggggtcaatg cactcggacg cggcggcggt gaggtcctgc aggtacaggc
     1741 tggaggtgga gcagacgctg tgcccgcggg cggggctcag gctggtgctg tctttgcgcg
     1801 cagcctggta ggaggccagc ttctccgaga ccagcttggc agcggctgag aaaccgctcc
     1861 acatacagtc ctggatgatg atgttcttga tgaaggtctc gtcgtcagga tcgcagatga
     1921 agctctggtt caccatgtct cctccaagta actcggtcat catctccagc tgatcggcgg
     1981 tggagaagtt gccaccgccg ccgtcatcgt cttcccttgg ggagaaggac gtagcgaccg
     2041 caacatagga tggagagcag agcccggagc ggcggctcgg ggacaggggc ggggtgggaa
     2101 gcagctcgaa tttcttccag atatcctcac tgggcgcggg cggctgcagc tcgctctgct
     2161 gttgctggtg atagaaattc tcttcctcgt cgcagatgaa atagggctgt acggagtcgt
     2221 agtcgaggtc atagttcctg ttggtgaagt tcacgttgag gggcatggtg aagggggcgg
     2281 ccgcggagcc tgcttttttg tacaaacttg tgatttcccg taccaccaca ctgggatcct
     2341 taattaacta gctagatccg gcagtctaga ggatggtcca cccccggggt cggcagcctt
     2401 cacgtgggcg gcgtgtatcc aagctgcgat gccgtctact ttgagggcgg tgggggtggt
     2461 cagcaggact gtgtaaggtc ctttccagcg aggttctagg ttcttagtct ggtgtcggcg
     2521 gacccacact gtgtcgccga ctcggtaagg gtgaggtacc accggtcggt ccagttgttc
     2581 ttggtaggct gccgccagag gtctccagac ttcgtgctgg actaagtaga gagcctgtaa
     2641 gtgagcttgg agagaggggc tgttagtaac tcttgtcatg tcagggtcag ggaagtttac
     2701 aaggggcggg ggtgccccat ataagatctc atatggccat atgggggcgc ctagagaagg
     2761 agtgagggct ggataaaggg aggatcgagg cggggtcgaa cgaggaggtt caagggggag
     2821 agacggggcg gatggaggaa gaggaggcgg aggcttaggg tgtacaaagg gcttgaccca
     2881 gggagggggg tcaaaagcca aggcttccca ggtcacgatg taggggacct ggtctgggtg
     2941 tccatgcggg ccaggtgaaa agaccttgat cttaacctgg gtgatgaggt ctcggttaaa
     3001 ggtgccgtct cgcggccatc cgacgttaaa ggttggccat tctgcagagc agaaggtaac
     3061 ccaacgtctc ttcttgacat ctaccgactg gttgtgagcg atccgctcga catctttcca
     3121 gtgacctaag gtcaaactta agggagtggt aacagtctgg ccctaatttt cagacaaata
     3181 cagaaacaca gtcagacaga gacaacacag aacgatgctg cagcagacaa gacgcgcggc
     3241 gcggcttcgg tcccaaaccg aaagcaaaaa ttcagacgga ggcgggaact gttttaggtt
     3301 ctcgtctcct accagaacca catatccctc ctattagggg ggtgcaccaa agagtccaaa
     3361 acgatcggga tttttggact caggtcgggc cacaaaaacg gcccccgaag tccctgggac
     3421 gtctcccagg gttgcggccg ggtgttccga actcgtcagt tccaccacgg gtccgccaga
     3481 tacagagcta gttagctaac tagtaccgac gcaggcgcat aaaatcagtc atagacacta
     3541 gacaatcgga cagacacaga taagttgctg gccagcttac ctcccggtgg tgggtcggtg
     3601 gtccctgggc aggggtctcc cgatcccgga cgagccccca aatgaaagac ccccgctgac
     3661 gggtagtcaa tcactcagag gagaccctcc caaggaacag cgagaccaca agtcggatgc
     3721 aactgcaaga gggtttattg gatacacggg tacccgggcg actcagtcaa tcggaggact
     3781 ggcgccccga gtgaggggtt gtgggctctt ttattgagct cggggagcag aagcgcgcga
     3841 acagaagcga gaagcgaact gattggttag ttcaaataag gcacagggtc atttcaggtc
     3901 cttggggcac cctggaaaca tctgatggtt ctctagaaac tgctgagggc tggaccgcat
     3961 ctggggacca tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct
     4021 gtttggccca tattcagctg ttccatctgt tcctgacctt gatctgaact tttctattct
     4081 cagttatgta tttttccatg ccttgcaaaa tggcgttact taagctagct tgccaaacct
     4141 acaggtgggg tctttcatta tgcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4201 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4261 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4321 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4381 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4441 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4501 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4561 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4621 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4681 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4741 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4801 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4861 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4921 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4981 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5041 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5101 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5161 gaccaagttt actcatatat actttagatt gatttgcggc cggccgcaaa aggatctagg
     5221 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5281 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5341 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5401 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5461 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5521 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5581 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5641 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5701 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5761 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5821 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5881 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5941 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6001 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6061 gcgagtcagt gagcgaggaa gcggaag
//
