LOCUS       sequence-116018-        1068 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses gRNA against human CEBPB along with Cas9 with 2A GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: PX458_CEBPB_1
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             62..127
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             134..154
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggaccg gtgccaccat
       61 ggactataag gaccacgacg gagactacaa ggatcatgat attgattaca aagacgatga
      121 cgataagatg gccccaaaga agaagcggaa ggtcggtatc cacggagtcc cagcagccga
      181 caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg ccgtgatcac
      241 cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag
      301 catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac
      361 ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct
      421 gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga
      481 agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat
      541 cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact
      601 ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat
      661 caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga
      721 caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa
      781 cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct
      841 ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat
      901 tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc
      961 caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat
     1021 cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccga
//
