LOCUS       sequence-116312-        1415 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  bacterial expression of a fluorescent calcium reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: pRSET-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..1415
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(32..40,41..58,59..61,62..94,95..97,98..121,122..148,
                     149..205,206..211,212..481,482..499,500..934,935..940,
                     941..1384)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      128..137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     418..439
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(545..566)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(806..825)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatagatc tatgggttct catcatcatc atcatcatgg
       61 tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa
      121 ggatctcgcc accatggtcg actcatcacg tcgtaagtgg aataagacag gtcacgcagt
      181 cagagctata ggtcggctga gctcactcga gaacgtctat atcaaggccg acaagcagaa
      241 gaacggcatc aaggcgaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct
      301 cgcctaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
      361 ccactacctg agcgtgcagt ccaaactttc gaaagacccc aacgagaagc gcgatcacat
      421 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
      481 gggcggtacc ggagggagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      541 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      601 tgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      661 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
      721 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacat
      781 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      841 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      901 cggcaacatc ctggggcaca agctggagta caacctgccg gaccaactga ctgaagagca
      961 gatcgcagaa tttaaagagg aattctccct atttgacaag gacggggatg ggacaataac
     1021 aaccaaggag ctggggacgg tgatgcggtc tctggggcag aaccccacag aagcagagct
     1081 gcaggacatg atcaatgaag tagatgccga cggtgacggc acaatcgact tccctgagtt
     1141 cctgacaatg atggcaagaa aaatgaaata cagggacacg gaagaagaaa ttagagaagc
     1201 gttcggtgtg tttgataagg atggcaatgg ctacatcagt gcagcagagc ttcgccacgt
     1261 gatgacaaac cttggagaga agttaacaga tgaagaggtt gatgaaatga tcagggaagc
     1321 agacatcgat ggggatggtc aggtaaacta cgaagagttt gtacaaatga tgacagcgaa
     1381 gtgaaagctt gatccggctg ntaacaaagc ccgaa
//
