LOCUS       sequence-116585-         794 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Godar S, Ince TA, Bell GW, Feldser D, Donaher JL, Bergh J, Liu A, 
            Miu K, Watnick RS, Reinhardt F, McAllister SS, Jacks T, Weinberg RA
  TITLE     Growth-inhibitory and tumor- suppressive functions of p53 depend on 
            its repression of CD44 expression.
  JOURNAL   Cell. 2008 Jul 11. 134(1):62-73.
  PUBMED    18614011
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Jul 11. 134(1):62-73."
COMMENT     Sequence Label: shCD44-3 pRRL
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    127..244
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(342..590)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(400..419)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(570..590)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ttccggaacc acgcccagag cagccgcgtt ccctgcgcaa acccagggct gccttggaaa
       61 aggcgcaacc ccaaccccct cgagccgcgg ccaaagtgga tctctgctgt ccctgtaata
      121 aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt
      181 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt
      241 aaaattgtgg atgaatactg ccatttgtct cgaggtcgag aattcaaaaa tgtaacacct
      301 acaccattat cctcgaggat aatggtgtag gtgttacaac cggtgtttcg tcctttccac
      361 aagatatata aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc
      421 attttaaaac ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac
      481 gtattttgta ctaatatctt tgtgtttaca gtcaaattaa ttccaattat ctctctaaca
      541 gccttgtatc gtatatgcaa atatgaagga atcatgggaa ataggccctc ggtgaagggg
      601 gcggccgctc gaggctagtc tcgtgatcga taccgtcgag atccgttcac taatcgaatg
      661 gatctgtctc tgtctctctc tccaccttct tcttctattc cttcgggcct gtcgggtccc
      721 ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc
      781 actatagaaa gtac
//
