LOCUS       sequence-116627-         685 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses CAS9-eGFP fusion protein in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Fujii W, Kawasaki K, Sugiura K, Naito K
  TITLE     Efficient generation of large-scale genome-modified mice using gRNA 
            and CAS9 endonuclease.
  JOURNAL   Nucleic Acids Res. 2013 Aug 30.
  PUBMED    23997119
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Aug 30."
COMMENT     Sequence Label: pCAG-T3-hCASeGFP-pA
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctcactcta gcgtctccac cgagctgaga gaggtcgatt cttgtttcat agagccccgt
       61 aattgactga tgaatcagtg tggcgtccag gacctccttt gtagaggtgt accgctttct
      121 gtctatggtg gtgtcgaagt acttgaaggc tgcaggcgcg cccaagttgg tcagagtaaa
      181 caagtggata atgttttctg cctgctccct gatgggctta tccctgtgct tattgtaagc
      241 agaaagcacc ttatcgaggt tagcgtcggc gaggatcact cttttggaga attcgcttat
      301 ttgctcgatg atctcatcaa ggtagtgttt gtgttgttcc acgaacagct gcttctgctc
      361 attatcttcg ggagaccctt tgagcttttc atagtggctg gccagataca agaaattaac
      421 gtatttagag ggcagtgcca gctcgttacc tttctgcagc tcgcccgcac tagcgagcat
      481 tcgtttccgg ccgttttcaa gctcaaagag agagtacttg ggaagcttaa tgatgaggtc
      541 ttttttgacc tctttatatc ctttcgcctc gagaaagtcg atggggtttt tttcgaagct
      601 tgatcgctcc atgattgtga tgcccagcag ttccttgacg cttttgagtt ttttagactt
      661 ccctttctcc actttggcca caacc
//
