LOCUS       sequence-116744-         707 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  YFP fusion of Sec23A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Stephens DJ, Lin-Marq N, Pagano A, Pepperkok R, Paccaud JP
  TITLE     COPI-coated ER-to-Golgi transport complexes segregate from COPII in 
            close proximity to ER exit sites.
  JOURNAL   J Cell Sci. 2000 Jun;113 ( Pt 12):2177-85.
  PUBMED    10825291
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2000-06"; volume: "113 ( Pt 12)"; pages: "2177-85"
COMMENT     Sequence Label: EYFP-Sec23A
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(52..54,55..57,58..570)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(97..118)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(358..377)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcagagctgg ttagtgaacc gtcagatccg ctagcgctac cggtcgccac catggtgagc
       61 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      121 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      181 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      241 accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
      301 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      361 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      421 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
      481 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
      541 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
      601 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      661 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacat
//
