LOCUS       sequence-117133-         810 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  expressses V5- and SBP-tagged RRP4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     Izaurralde lab Exosc plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pT7-V5-SBP-C1-HsRRP4
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..132
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             154..267
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWGGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctctag cgctaccggt cgccaccatg ggtaagccta tccctaaccc tctcctcggt
      121 ctcgattcta cgcccggaag cggtagcggt acgatggacg agaagaccac cggctggggg
      181 ggcggccacg tggtggaggg cctggccggc gagctggagc agctgcgggc caggctggag
      241 caccaccctc agggccagcg ggagcccaga tctcgaggga tggcgatgga gatgaggctt
      301 ccagtggctc gcaagcctct tagcgagaga ctgggccgcg acactaagaa acatctagtg
      361 gtgccggggg atacaatcac tacggacaca ggattcatgc ggggccatgg aacgtatatg
      421 ggagaagaga agctcattgc atctgttgct ggctctgtgg agagagtaaa caagttgatc
      481 tgtgtgaaag ctttgaaaac cagatacatt ggtgaagtag gagacatcgt agtgggacga
      541 atcacagagg ttcaacagaa gaggtggaag gtggagacca actccaggct ggattcggtc
      601 ttgctgctct cgtccatgaa ccttcctgga ggagagctga ggagaagatc tgcagaagat
      661 gagcttgcaa tgagaggttt cttacaggaa ggggacctta tcagtgctga ggtccaggca
      721 gtgttctctg acggagctgt ctctttgcac acgaggagcc tgaaatatgg aaaactaggt
      781 cagggggttt tggtccaggt ttccccctcc
//
