LOCUS       sequence-117156-        1114 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: BB3-Tango
FEATURES             Location/Qualifiers
     source          1..1114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact
      121 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgcgc caccatgaag
      181 acgatcatcg ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat
      241 gacgccagca tcgatatggc tcaaaggcag cctcactcac ctaatcagac tttaatttca
      301 atcacaaatg acacagaatc atcaagctct gtggtttcta acgataacac aaataaagga
      361 tggagcgggg acaactctcc aggaatagaa gcattgtgtg ccatctatat tacttatgct
      421 gtgatcattt cagtgggcat ccttggaaat gctattctca tcaaagtctt tttcaagacc
      481 aaatccatgc aaacagttcc aaatattttc atcaccagcc tggcttttgg agatctttta
      541 cttctgctaa cttgtgtgcc agtggatgca actcactacc ttgcagaagg atggctgttc
      601 ggaagaattg gttgtaaggt gctctctttc atccggctca cttctgttgg tgtgtcagtg
      661 ttcacattaa caattctcag cgctgacaga tacaaggcag ttgtgaagcc acttgagcga
      721 cagccctcca atgccatcct gaagacttgt gtaaaagctg gctgcgtctg gatcgtgtct
      781 atgatatttg ctctacctga ggctatattt tcaaatgtat acacttttcg agatcccaat
      841 aaaaatatga catttgaatc atgtacctct tatcctgtct ctaagaagct cttgcaagaa
      901 atacattctc tgctgtgctt cttagtgttc tacattattc cactctctat tatctctgtc
      961 tactattcct tgattgctag gaccctttac aaaagcaccc tgaacatacc tactgaggaa
     1021 caaagccatg cccgtaagca gattgaatcc cgaaagagaa ttgccagaac ggtattggtg
     1081 ttggtggctc tgtttgccct ctgctggttg ccaa
//
