LOCUS       sequence-117225-         911 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses mCherry under the control of Mutant T7 Promoter C4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Jones JA, Vernacchio VR, Lachance DM, Lebovich M, Fu L, Shirke AN, 
            Schultz VL, Cress B, Linhardt RJ, Koffas MA
  TITLE     ePathOptimize: A Combinatorial Approach for Transcriptional 
            Balancing of Metabolic Pathways.
  JOURNAL   Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
  PUBMED    26062452
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-11"; volume: "5:11301. doi"; pages: " 
            10.1038/srep11301"
COMMENT     Sequence Label: pETM6-C4-mCherry
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    75..99
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             114..136
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gccacgatgc gtccggcgta gcctaggatc gagatcgatc tcgatcccgc gaaattaata
       61 cgactcacta tcaaggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt
      121 aactttaaga aggagatata catatggttt caaaaggcga agaagacaac atggcgatta
      181 tcaaggaatt tatgcgtttc aaggtccaca tggaaggcag cgtcaatggt cacgaatttg
      241 aaattgaagg cgaaggtgaa ggccgtccgt atgaaggcac ccagacggca aaactgaagg
      301 tcaccaaagg cggtccgctg ccgtttgctt gggatattct gtcaccgcaa ttcatgtatg
      361 gttcgaaagc gtacgttaag catccggccg atatcccgga ctatctgaaa ctgtcctttc
      421 cggaaggctt caaatgggaa cgtgttatga acttcgaaga tggcggtgtg gttaccgtca
      481 cgcaggatag ctctctgcaa gacggtgaat ttatttataa agtgaagctg cgcggcacca
      541 atttcccgag cgatggtccg gttatgcaga aaaagacgat gggctgggaa gcgagttccg
      601 aacgtatgta cccggaagac ggtgccctga aaggcgaaat caagcagcgc ctgaaactga
      661 aggatggcgg tcactatgac gcagaagtga aaaccacgta caaggctaaa aagccggtcc
      721 aactgccggg tgcatacaac gtgaacatca agctggatat caccagccat aacgaagact
      781 atacgatcgt tgaacagtac gaacgtgcag aaggccgcca ctctaccggc ggtatggatg
      841 aactgtacaa ataaggtacc ctcgagtctg gtaaagaaac cgctgctgcg aaatttgaac
      901 gccagcacat g
//
