LOCUS       sequence-117338-         931 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Adenoviral vector for the expression of the Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han YC,
            Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura
            A
  TITLE     In vivo engineering of oncogenic chromosomal rearrangements with the
            CRISPR/Cas9 system.
  JOURNAL   Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 
            2014 Oct 22.
  PUBMED    25337876
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13902"; journalName: "Nature"; date: "2014-12-18- 
            18"; volume: "516"; issue: "7531"; pages: "423-7"
COMMENT     Sequence Label: Adeno Cas9
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        161..409
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     161..181
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     332..351
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        428..503
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        600..885
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 gatgttgtag taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg
       61 aataagagga agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta
      121 gggagatccg gtaccgttta aactcgagaa tggcgcatgt gagggcctat ttcccatgat
      181 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
      241 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      301 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
      361 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg ggtcttcgag
      421 aagacctgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
      481 aaaaagtggc accgagtcgg tgcttttttg ttttagagct agaaatagca agttaaaata
      541 aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac aaatggctct agaggtaccc
      601 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      661 acgtcaatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      721 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      781 gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt acatgacctt atgggacttt
      841 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
      901 acgttctgct tcactctccc catctccccc c
//
