LOCUS       sequence-117442-        3261 bp    DNA     circular SYN 15-APR-2025
DEFINITION  OR2-OR1-Pr promoter expressing deCFP-MG aptamer fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Siegal-Gaskins D, Tuza ZA, Kim J, Noireaux V, Murray RM
  TITLE     Gene circuit performance characterization and resource usage in a 
            cell-free ""breadboard"".
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. 
            Epub 2014 Apr 7.
  PUBMED    24670245
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400203p";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "416-25"
COMMENT     Sequence Label: Pr-deCFP-MGapt
FEATURES             Location/Qualifiers
     source          1..3261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(232..336)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     404..422
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             760..782
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(819..840)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1080..1099)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1427..1448
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1702..1719)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1873..2461)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1953..1972)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2632..3261,1..162,163..231))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     join(3255..3261,1..13)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
       61 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
      121 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
      181 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
      241 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
      301 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
      361 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
      421 gccctttcgt cttcaagaat tctggcgaat cctctgacca gccagaaaac gacctttctg
      481 tggtgaaacc ggatgctgca attcagagcg ccagcaagtg ggggacagca gaagacctga
      541 ccgccgcaga gtggatgttt gacatggtga agactatcgc accatcagcc agaaaaccga
      601 attttgctgg gtgggctaac gatatccgcc tgatgcgtga acgtgacgga cgtaaccacc
      661 gcgacatgtg tgtgctgttc cgctgggcat gctgagctaa caccgtgcgt gttgacaatt
      721 ttacctctgg cggtgataat ggttgcagct agcaataatt ttgtttaact ttaagaagga
      781 gatataccat ggagcttttc actggcgttg ttcccatcct ggtcgagctg gacggcgacg
      841 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      901 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      961 ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     1021 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1081 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1141 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1201 agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     1261 aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1321 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1381 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1441 agttcgtgac cgccgccggg atctaaacaa taactgaata ggggatcccg actggcgaga
     1501 gccaggtaac gaatggatcc ccgagctcga gcaaagcccg ccgaaaggcg ggcttttctg
     1561 tgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     1621 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     1681 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1741 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1801 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1861 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1921 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1981 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2041 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2101 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2161 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2221 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2281 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2341 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2401 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2461 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2521 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2581 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2641 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2701 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2761 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2821 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2881 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2941 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3001 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3061 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3121 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3181 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3241 ggcgtcaaca cgggataata c
//
