LOCUS       sequence-117605-         858 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Mitochondria, Excitation: 598, Emission: 625.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRaspberry-Mito-7
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..104
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             138..818
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mRaspberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQCMYGSKGYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     complement(271..289)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     539..558
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     733..756
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tggtttagtg aaccgtcaga tccgctagca tgtccgtcct gacgccgctg ctgctgcggg
       61 gcttgacagg ctcggcccgg cggctcccag tgccgcgcgc caagatccat tcgttggggg
      121 atccaccggt ggctaccatg gtgagcaagg gcgaggaggt catcaaggag ttcatgcgct
      181 tcaaggtgcg catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
      241 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
      301 tgcccttcgc ctgggacatc ctgtcccctc agtgcatgta cggctccaag ggctacgtga
      361 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
      421 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
      481 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
      541 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
      601 acggcgccct gaagggcgag atgaagatga ggctgaagct gaaggacggc ggccactacg
      661 acgccgaggt caagaccacc tacatggcca agaagcccgt gcagctgccc ggcgcctaca
      721 agaccgacat caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt
      781 acgagcgcgc cgagggccgc cactccaccg gcgcctaagc ggccgcgact ctagatcata
      841 atcagccata ccacattt
//
